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Information on EC 3.2.1.14 - chitinase
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3.2.1.14 chitinaseIUBMB Comments
The enzyme binds to chitin and randomly cleaves glycosidic linkages in chitin and chitodextrins in a non-processive mode, generating chitooligosaccharides and free ends on which exo-chitinases and exo-chitodextrinases can act. Activity is greatly stimulated in the presence of EC 1.14.99.53, lytic chitin monoxygenase, which attacks the crystalline structure of chitin and makes the polymer more accesible to the chitinase. cf. EC 3.2.1.202, endo-chitodextrinase.
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Word Map
- 3.2.1.14
-
antifungal
-
colloidal
-
biocontrol
- trichoderma
-
chitin-binding
- fusarium
-
pathogenesis-related
- serratia
- marcescens
- gaucher
-
freezing
- n-acetylglucosamine
- solani
-
3-like
- beta-1,3-glucanase
- glcnac
-
phytopathogenic
- cellulase
- rhizoctonia
- cinerea
- harzianum
- glucanase
- oxysporum
- botrytis
-
defense-related
- mycelial
- colletotrichum
- chitooligosaccharides
-
hysteresis
-
rhizosphere
- cuticle
-
molt
- alternaria
- circulans
- n-acetyl-d-glucosamine
- flounder
-
entomopathogenic
- chitosanase
-
anthracnose
- beauveria
- viride
-
recrystallization
- pythium
- glucocerebrosidase
- bassiana
- metarhizium
- nucleopolyhedrovirus
-
4-methylumbelliferyl
-
transglycosylation
- sclerotiorum
- biotechnology
- degradation
- medicine
- analysis
- industry
- synthesis
- pharmacology
- molecular biology
- environmental protection
- agriculture
The enzyme appears in viruses and cellular organisms
Synonyms
chitinase, chitotriosidase, endochitinase, antifreeze protein, chit1, amcase, chitinase a, exochitinase, acidic mammalian chitinase, class i chitinase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AcD1ChiA
acidic chitinase
acidic mammalian chitinase
alpha-chitinase
AMCase
BJL200-ChiC1
CHI
Chi-h
Chi18C
CHI2
Chi35
Chi40
Chi44
Chi46
Chi54
CHI60
Chi67
Chi70
Chi72
Chi78
ChiA
ChiA-Hh59
ChiA-Mt45
ChiA74
ChiB
ChiC
ChiCW
ChiD
ChiE
ChiEN1
ChiF
ChiF1
ChiG
ChiNCTU2
Chit
CHIT1
CHIT42
Chit62
ChitA
chitinase 1
chitinase 18C
chitinase 2
chitinase 60
chitinase 92
chitinase A
chitinase A1
chitinase B
chitinase C
chitinase C1
chitinase F1
chitinase G
chitinase I
chitinase III
chitinase-A
chitinases A
chitinolytic enzyme
ChiW
CHT1
class I chitinase
class III chitinase
class IV chitinase
class V chitinase
ECH
Ech42
endo-chitinase
endochitinase
endochitinase-1
endochitinase-2
HschiA1
Is-chiB
LpChiA
poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase
PrChi-A
Ptchi19p
Ra-ChiC
Tfu_0580
TKU028 chitinase
additional information
poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase
-
-
additional information
PLChiA belongs to the class III chitinases in glycoside hydrolase family 18
additional information
-
the enzyme belongs to the family 19 of glycosyl hydrolases, GH19
additional information
-
sAMC belongs to glycosyl hydrolase family 19 chitinases which are mostly endochitinases
additional information
-
the enzyme is a GH18 family chitinase
additional information
the enzyme belongs to family 19 of the glycosyl hydrolases
additional information
ChiA belongs to family 19 of glycosyl hydrolases
additional information
the isozyme belongs to the glycoside hydrolase family 48, GH48
additional information
the isozyme belongs to the glycoside hydrolase family 48, GH48
additional information
AMCase is a glyco_18_domain-containing protein and a member of glycoside hydrolase family 18
additional information
the endochitinase belongs to the glycosyl hydrolase family 19
additional information
th enzyme belongs to the family 18 of glycohydrolases
additional information
-
OsChia1b is a class I chitinase of glucohydrolase family 19
additional information
-
the chitin-binding domain 2 belongs to the carbohydrate binding module 2 family of proteins
additional information
ChiB belongs to the family 18 of glycohydrolases
additional information
BJL200-ChiC1 belongs to the family 18 chitinases
additional information
BJL200-ChiC1 belongs to the family 18 chitinases
additional information
-
the enzyme belongs to family 18 of glycosyl hydrolases
additional information
-
the enzyme belongs to family 18 of glycosyl hydrolases
additional information
-
ChiG belongs to the bacterial family 19 of glycohydrolases
additional information
-
the enzymes belong to the chitinase families 18, Chi18aC and Chi18aD, and family 19, isozymes Chi18bA and Chi19F
additional information
-
ChiG belongs to the bacterial family 19 of glycohydrolases
additional information
-
the enzymes belong to the chitinase families 18, Chi18aC and Chi18aD, and family 19, isozymes Chi18bA and Chi19F
additional information
-
the enzyme belongs to the acidic class III chitinase family
additional information
-
chitinase A belongs to the glycosyl hydrolase family 18
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
-
-
-
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
catalytic reaction mechanism involving Glu119, overview
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
exo-type substrate cleavage mechanism
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
reaction mechanism, Glu315 is the acid/base during hydrolysis, the side-chain of the preceding Asp313 helps to position the N-acetyl moiety of the glycosyl sugar undergoing hydrolysis correctly, mutation of the conserved Tryp167 in the chitin-binding cleft of chitinase A enhances transglycosylation, transglycosylation reaction scheme, overview
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
the enzyme shows endo- and exo-hydrolytic activity, as well as transglycosylation activity
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
catalytic mechanism, overview
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
catalytic mechanism with an important role of Trp-33 on enzyme processivity, CHI60 preferentially uses the endo-type mode on soluble and amorphous substrates and the exo-type mode on crystalline substrate, the enzyme hydrolyzes crystalline substrates with an exo-type mode processively while remaining tightly bound to the substrate. The prevalent mode of hydrolysis mediated by CHI60 is regulated by ionic strength
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
chitooligosaccharide-binding modes and catalytic mechanism, overview
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
mode of action of ChiG, family 19 chitinases use a single-displacement reaction with inversion of the configuration of the anomeric carbon, overview
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
double displacement reaction mechanism, overview
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
mode of action of ChiG, family 19 chitinases use a single-displacement reaction with inversion of the configuration of the anomeric carbon, overview
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
double displacement reaction mechanism, overview
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
exo-type substrate cleavage mechanism
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
catalytic mechanism with an important role of Trp-33 on enzyme processivity, CHI60 preferentially uses the endo-type mode on soluble and amorphous substrates and the exo-type mode on crystalline substrate, the enzyme hydrolyzes crystalline substrates with an exo-type mode processively while remaining tightly bound to the substrate. The prevalent mode of hydrolysis mediated by CHI60 is regulated by ionic strength
-
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
the enzyme shows endo- and exo-hydrolytic activity, as well as transglycosylation activity
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
(1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase
The enzyme binds to chitin and randomly cleaves glycosidic linkages in chitin and chitodextrins in a non-processive mode, generating chitooligosaccharides and free ends on which exo-chitinases and exo-chitodextrinases can act. Activity is greatly stimulated in the presence of EC 1.14.99.53, lytic chitin monoxygenase, which attacks the crystalline structure of chitin and makes the polymer more accesible to the chitinase. cf. EC 3.2.1.202, endo-chitodextrinase.
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CAS REGISTRY NUMBER
COMMENTARY
9001-06-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(GlcNAc)6 + H2O
(GlcNAc)2 + (GlcNAc)3 + (GlcNAc)4
the enzyme prefers the beta-anomer of chito-oligosaccharides as substrate, cleavage of beta-glycosidic linkages from the non-reducing end primarily by disaccharide units
product analysis
r
2-acetamido-2-deoxy-4-O-(2-acetamido-2-deoxy-4-O-methyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-acetamido-4-O-cyclohexylmethyl-2-deoxy-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-deoxy-2-acetamido-4-O-isopropyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside + H2O
?
-
-
-
?
4-methylumbelliferyl 2-acetamido-4-O-(2-acetamido-2,4-dideoxy-beta-D-xylo-hexopyranosyl)-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferol + 2-acetamido-4-O-(2-acetamido-2,4-dideoxy-beta-D-xylo-hexopyranosyl)-2-deoxy-beta-D-glucopyranoside
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-triacetylchitotriose
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferone + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-D-N,N'-diacetylchitobioside
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O
4-methylumbelliferol + beta-N-acetyl-D-glucosamine
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
4-methylumbelliferyl N,N',N''-beta-D-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-beta-D-triacetylchitotriose
substrate is prefered over shorter GlcNAc-substrates
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
4-methylumbelliferyl N,N'-diacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
4-methylumbelliferyl-D-N,N'-diacetylchito-bioside + H2O
4-methylumbelliferone + D-N,N'-diacetylchito-bioside
-
-
-
?
4-nitrophenyl beta-1,4-N,N'-diacetyl-chitobiose + H2O
4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose
-
-
?
4-nitrophenyl beta-D- N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide
-
535% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
-
-
-
?
4-nitrophenyl beta-D-GlcNAc + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
0.8% of the activity with 4-nitrophenyl-(beta-D-GlcNAc)3
-
?
4-nitrophenyl beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetyl-beta-D-glucosamine
-
700% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide
-
-
?
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + 3 H2O
4-nitrophenol + 2 N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenol + beta-N,N',N'',N'''-tetraacetylchitotetraose
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-N,N',N''-triacetylchitotriose
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + beta-N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
4-nitrophenyl N,N',N''-beta-D-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-beta-D-triacetylchitotriose + N,N'-diacetyl-beta-D-chitobiose + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-nitrophenyl N-acetyl-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
?
4-nitrophenyl N-acetylchitobioside + H2O
4-nitrophenol + N-acetylchitobiose
-
-
-
?
4-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-nitrophenol + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
?
alpha-chitin + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
alpha-type chitosan + H2O
?
-
98% deacetylated, 18% of the activity with colloidal chitin
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
beta-D-N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
-
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
-
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
86% activity compared to colloidal chitin
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
beta-D-N,N'-diacetylchitobiose + ?
-
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
?
beta-type chitin + H2O
?
-
best substrate, 148% of the activity with colloidal chitin
-
?
beta-type chitosan + H2O
?
-
98% deacetylated, 26% of the activity with colloidal chitin
-
?
chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine
chitin + H2O
N,N-diacetylchitobiose + N-acetylD-glucosamine
-
-
?
chitin flakes + H2O
N-acetyl-beta-D-glucosamine + ?
-
10.8% activity compared to colloidal chitin
-
?
chitin oligosaccharides + H2O
N-acetyl glucosamine
glycol chitin assay method, (GlcNAc)1-6
-
?
chitin powder + H2O
N-acetyl-beta-D-glucosamine + ?
-
32.5% activity compared to colloidal chitin
-
?
chitohexaose + H2O
2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose
reaction via trimer formation
-
?
chitohexaose + H2O
chitotetraose + chitobiose
-
the enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)6
-
?
chitopentaose + H2O
N-acetylglucosamine + 2 N,N'-diacetylchitobiose
reaction via trimer formation
-
?
chitosan + H2O
glucosamine + chitobiose + chitooligomers with a chain length longer than 6
-
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + ?
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
colloidal chitin + H2O
chitobiose + D-glucosamine
from crab shell
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetyl D-glucosamine
-
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + chitobiose + chitotriose
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + tetra-N-acetyl-beta-D-glucosamine
100% activity
-
?
crab shell chitin + H2O
?
the enzyme cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly
-
?
crystalline alpha-chitin + H2O
GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)-GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta
-
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNACbeta(1-4)GlcNAcbeta
-
-
some (GlcNAc)3 is produced through transglycosylation reaction
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAc
-
i.e. (GlcNAc)6
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
i.e. (GlcNAc)6
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
-
-
product distribution is (GlcNAc)3 >(GlcNAc)4 > (GlcNAc)2 for the wild type enzyme. Some (GlcNAc)5 is produced, without formation of GlcNAc, suggesting that it is not directly produced from the initial substrate (GlcNAc)6 but produced through transglycosylation reaction
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
glycol chitin + H2O
N,N'-diacetylchitobiose + GlcNAc
-
exochitinase activity that yields p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose, and then when the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is accumulated, the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is further cleaved into N,N'-diacetylchitobiose and GlcNAc
-
?
glycol chitin + H2O
N,N'-diactylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
-
-
?
hexa-N-acetyl-chitohexaose + H2O
2 tri-N-acetyl-chitotriose
highest activity
-
?
N,N',N'''-triacetyl-beta-D-chitotriose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
-
a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant. BcChi-A catalyzes an anomer inversion reaction
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
the enzyme hydrolyzes the third and fourth glycosidic linkages from the non-reducing end of N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose
-
?
N,N',N'',N''', N''''-pentaacetylchitopentaose + H2O
5 N-acetyl-D-glucosamine
-
-
?
N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose + H2O
?
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N''',N''''-pentaacetylchitopentaose
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
wild-type enzyme: the alpha/beta ratios of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose are 26.8 and 2.95, respectively
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity
?
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
N,N',N'',N''',N'''',N'''''-pentacetyl-beta-D-chitopentaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
(GlcNAc)2 + (GlcNAc)3
-
-
a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
major product
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N-acetylglucosamine + N,N'-diacetylchitobiose
reaction via trimer formation
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
4 N-acetyl-beta-D-glucosamine
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + ?
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose
-
-
transglycosilation activity
?
N,N',N'',N'''-tetracetyl-beta-D-chitotetraose + H2O
N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
N,N',N''-triacetyl-beta-D-chitotriose + H2O
N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
?
N,N',N''-triacetylchitotriose + 2 H2O
3 beta-N-acetyl-D-glucosamine
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetylglucosamine
-
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + GlcNAc
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-cellobiose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
D-galactopyranose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
D-glucopyranose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-lactopyranoside + H2O
D-lactopyranose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-maltoside + H2O
D-maltose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
D-mannopyranose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
N,N',N''-triacetylchitotriose + p-nitrophenol
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc
-
-
?
p-nitrophenyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
?
p-nitrophenyl-N,N',N''-triacetylchitotriose + H2O
p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc
-
-
?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
partially deacetylated chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
tri-N-acetyl-chitotriose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
substrate for isoform Chi78
-
?
3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O
?
-
-
-
?
3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
-
product analysis
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
product analysis
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl-(GlcNAc) + H2O
4-methylumbelliferone + N-acetyl-D-glucosamine
-
-
?
4-methylumbelliferyl-(GlcNAc) + H2O
4-methylumbelliferone + N-acetyl-D-glucosamine
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
substrate concentration of 500 microM in citrate buffer
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
chit37 and chit42
-
?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
250% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
198% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
highest activity
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
highest activity
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
185% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
150% activity compared to colloidal chitin
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Salmonella enterica typhimurium
-
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
highest activity
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Thermobifida fusca ATCC BAA-629
highest activity
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
essential role of the two carboxylic acid residues E172 and D170 in the catalytic mechanism of Chit42
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
?
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
synthetic substrate
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
best substrate
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
best substrate
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
p-nitrophenol + N-acetyl-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
p-nitrophenol + N-acetyl-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
-
-
?
4-nitrophenyl tri-N-acetylchitotrioside + H2O
4-nitrophenol + tri-N-acetylchitotriose
-
-
-
?
4-nitrophenyl tri-N-acetylchitotrioside + H2O
4-nitrophenol + tri-N-acetylchitotriose
-
-
-
?
alpha-chitin + H2O
?
-
strain LMG 11386 shows low enzyme activity
-
?
alpha-chitin + H2O
?
2.1% activity compared to colloidal chitin
-
?
alpha-crystal chitin + H2O
di-N-acetyl-chitobiose
-
-
?
alpha-type chitin + H2O
?
-
99% of the activity with colloidal chitin
-
?
beta-1,4-N-acetylglucosamine polymer + H2O
?
-
linear polymer, endo- and exo-hydrolytic activity
-
?
beta-1,4-N-acetylglucosamine polymer + H2O
?
-
linear polymer, endo- and exo-hydrolytic activity
-
?
beta-chitin + H2O
?
4.3% activity compared to colloidal chitin
-
?
beta-chitin + H2O
?
substate 3 micrometer beta-chitin
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose
best substrate
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose
Paenibacillus barengoltzii CAU904
best substrate
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose
Paenibacillus barengoltzii CAU904
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose
-
-
?
carboxymethyl cellulose + H2O
?
-
1.9% of the activity with colloidal chitin
-
?
carboxymethyl cellulose + H2O
?
3.5% activity compared to colloidal chitin
-
?
carboxymethyl cellulose + H2O
?
Paenibacillus barengoltzii CAU904
3.5% activity compared to colloidal chitin
-
?
carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
?
-
-
-
?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
?
-
-
?
carboxymethylated-chitin-remazol brilliant violet + H2O
?
-
isoenzymes I and II
-
?
cell wall + H2O
chitin oligosaccharides
-
from Rhizopus delemar
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
PLC-B hydrolyses Micrococcus lysodeikticus cell wall
-
?
cell wall + H2O
chitin oligosaccharides
-
from yeast
N-acetetylglucosamine and diacetylchitobiose
?
cell wall + H2O
chitin oligosaccharides
-
fungal, Phytium and Rhizoctonia solani
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
fungal, Phytium and Rhizoctonia solani
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
chit42, cell wall from Botrytis cinerea
-
?
chitin + H2O
?
-
high activity, the enzyme of strain 16-3 is active with colloidal chitin and mycelia of Trichoderma virens, Mucor hiemalis strain Bu 10, and Trichoderma harzianum strain Mn 75, overview
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
?
chitin + H2O
?
-
swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin
-
?
chitin + H2O
?
-
swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin
-
?
chitin + H2O
?
-
the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C
-
?
chitin + H2O
?
-
the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C
-
?
chitin + H2O
?
-
colloidal , regenerated, or partially N-acetylated chitin substrates
-
?
chitin + H2O
?
-
optimal substrate concentration is 0.6 mg and 0.4 mg enzyme per reaction mixture
-
?
chitin + H2O
?
-
colloidal , regenerated, or partially N-acetylated chitin substrates
-
?
chitin + H2O
?
-
the enzyme plays a role in pathogen defense and serves as biocontrol agent against the adult Japanese pine sawyer, Monochamus alternatus, oral ingestion of the enzyme by the insect causes high mortality, the enzyme degrades peritrophic membrane chitin in the beetle, but does not affect the midgut epithelium
-
?
chitin + H2O
?
-
colloidal and patially deacetylated chitin PC-100
-
?
chitin + H2O
?
-
colloidal and patially deacetylated chitin PC-100
-
?
chitin + H2O
?
cell wall chitin of Septoria tritici, Chi46 enzyme can also efficiently degrade cell walls of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici, and Phytophthora sojae
-
?
chitin + H2O
?
active toward soluble and insoluble chitin preparations
-
?
chitin + H2O
?
active toward soluble and insoluble chitin preparations
-
?
chitin + H2O
?
-
substrate of CHITI1 and AMCase, the polysaccharide is contained in many human parasites
-
?
chitin + H2O
?
a flexible loop controlling the enzymatic activity and specificity in the glycosyl hydrolase family 19 endochitinase from barley seeds
-
?