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Literature summary for 3.2.1.14 extracted from

  • Matsumiya, M.; Arakane, Y.; Haga, A.; Muthukrishnan, S.; Kramer, K.J.
    Substrate specificity of chitinases from two species of fish, greenling, Hexagrammos otakii, and common mackerel, Scomber japonicus, and the insect, tobacco hornworm, Manduca sexta (2006), Biosci. Biotechnol. Biochem., 70, 971-979.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for glycolchitin is 0.182 mg/ml Scomber japonicus
additional information
-
 additional information Km-value for glycolchitin is 0.471 mg/ml, isoenzyme HoChiB Hexagrammos otakii
additional information
-
 additional information Km-value for glycolchitin is 0.494 mg/ml, isoenzyme HoChiC Hexagrammos otakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, isoenzyme HoChiC, SDS-PAGE Hexagrammos otakii
51000
-
 x * 51000, isoenzyme HoChiB, SDS-PAGE Hexagrammos otakii
62000
-
 x * 62000, isoenzyme HoChiA, SDS-PAGE Hexagrammos otakii

Organism

Organism UniProt Comment Textmining
Hexagrammos otakii
-
-
-
Manduca sexta
-
-
-
Scomber japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme HoChiA Hexagrammos otakii
isoenzyme HoChiB Hexagrammos otakii
isoenzyme HoChiC Hexagrammos otakii

Source Tissue

Source Tissue Comment Organism Textmining
stomach isoenzyme HoChiA Hexagrammos otakii
-
stomach isoenzyme HoChiB Hexagrammos otakii
-
stomach isoenzyme HoChiC Hexagrammos otakii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.25
-
 isoenzyme HoChiB Hexagrammos otakii
1.25
-
 isoenzyme HoChiC Hexagrammos otakii
3.04
-
 isoenzyme HoChiA Hexagrammos otakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Scomber japonicus ?
-
 ?
chitin + H2O
-
 Manduca sexta ?
-
 ?
chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
glycolchitin + H2O
-
 Scomber japonicus ?
-
 ?
glycolchitin + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
glycolchitin + H2O isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
glycolchitin + H2O isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc) Scomber japonicus ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi386 Manduca sexta ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi535 Manduca sexta ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 13% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi535 Manduca sexta ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 27% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi386 Manduca sexta ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 30% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose Scomber japonicus ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O low activity, isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O low activity, isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O
-
 Scomber japonicus ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O enzyme form MsChi386 Manduca sexta ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O enzyme form MsChi535 Manduca sexta ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiC Hexagrammos otakii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, isoenzyme HoChiC, SDS-PAGE Hexagrammos otakii
? x * 51000, isoenzyme HoChiB, SDS-PAGE Hexagrammos otakii
? x * 62000, isoenzyme HoChiA, SDS-PAGE Hexagrammos otakii

Synonyms

Synonyms Comment Organism
HoChiA
-
 Hexagrammos otakii
HoChiB
-
 Hexagrammos otakii
HoChiC
-
 Hexagrammos otakii
MsChi386
-
 Manduca sexta
MsChi535
-
 Manduca sexta
SjChi
-
 Scomber japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 isoenzyme HoChiA, substrate: glycolchitin Hexagrammos otakii
70
-
 isoenzyme HoChiB, substrate: glycolchitin Hexagrammos otakii
70
-
 isoenzyme HoChiC, substrate: glycolchitin Hexagrammos otakii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 30% of maximal activity, 70°C: about 60% of maximal activity, isoenzyme HoChiA, substrate: glycolchitin Hexagrammos otakii
50 80 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity, isoenzyme HoChiB, substrate: glycolchitin Hexagrammos otakii
50 80 50°C: about 40% of maximal activity, 80°C: about 70% of maximal activity, isoenzyme HoChiC, substrate: glycolchitin Hexagrammos otakii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.06
-
 glycolchitin isoenzyme HoChiB Hexagrammos otakii
1.85
-
 glycolchitin isoenzyme HoChiC Hexagrammos otakii
2.21
-
 glycolchitin isoenzyme HoChiC Scomber japonicus
3.15
-
 glycolchitin isoenzyme HoChiA Hexagrammos otakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
 the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA Hexagrammos otakii
8
-
 isoenzyme HoChiB Hexagrammos otakii
8
-
 isoenzyme HoChiC Hexagrammos otakii
8
-
 the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA Hexagrammos otakii

pH Range

pH Minimum pH Maximum Comment Organism
1 8 pH 1.0: about 70% of maximal activity, pH 6.0: 15% of maximal activity, pH 8.0: about 35% of maximal activity Hexagrammos otakii
1 9 HoChiB has more than 50% activity from pH 1 to 9, and the highest activity is at pH 8 Hexagrammos otakii
1 9 HoChiC has more than 50% activity from pH 1 to 9, and the highest activity was at pH 8 Hexagrammos otakii

pI Value

Organism Comment pI Value Maximum pI Value
Hexagrammos otakii isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiA
-
 5.7
Hexagrammos otakii isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiB
-
 7.6
Hexagrammos otakii isoelectric focusing, pH range of 3.5–9.5, isoenzyme HoChiC
-
 8.8