KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | at pH 7.0 and 60°C | Paenibacillus thermoaerophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus thermoaerophilus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + H2O | Paenibacillus thermoaerophilus | - |
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose | - |
? | |
colloidal chitin + H2O | Paenibacillus thermoaerophilus TC22-2b | - |
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus thermoaerophilus | - |
- |
- |
Paenibacillus thermoaerophilus TC22-2b | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, colloidal chitin adsorption, and DEAE Sepharose column chromatography | Paenibacillus thermoaerophilus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
colloidal chitin + H2O | - |
Paenibacillus thermoaerophilus | N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose | - |
? | |
colloidal chitin + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose | - |
? | |
additional information | the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose | Paenibacillus thermoaerophilus | ? | - |
? | |
additional information | the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose | Paenibacillus thermoaerophilus TC22-2b | ? | - |
? | |
N,N',N'''-triacetyl-beta-D-chitotriose + H2O | - |
Paenibacillus thermoaerophilus | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'''-triacetyl-beta-D-chitotriose + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O | - |
Paenibacillus thermoaerophilus | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'',N''',N'''',N'''''-pentacetyl-beta-D-chitopentaose + H2O | - |
Paenibacillus thermoaerophilus | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'',N'''-tetracetyl-beta-D-chitotetraose + H2O | - |
Paenibacillus thermoaerophilus | N,N'-diacetyl-beta-D-chitobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Paenibacillus thermoaerophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Paenibacillus thermoaerophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | more than 69% of the maximum activity is observed in the temperature range of 50-70°C. Enzyme activity increases from 30 to 60°C and then declined markedly at temperature of more than 70°C | Paenibacillus thermoaerophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | the enzyme has no activity when incubated at 80°C for 2 h. However, it retains more than 68 % of its initial activity after incubation at 30, 40, and 50°C for 2 h. This enzyme also retains more than 47 % of its initial activity after incubation at 30, 40, and 50°C for 24 h | Paenibacillus thermoaerophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.6 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | at pH 7.0 and 60°C | Paenibacillus thermoaerophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Paenibacillus thermoaerophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | more than 50% of its maximum activity is observed in the pH range of 4.0-7.0 | Paenibacillus thermoaerophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme retains more than 80% activity after incubation at 30°C for 2 h in the pH range of 4.0-10.0. Residual activity is reduced at an acidic pH, in particular, approximately 60% of its activity is lost at pH 3.0 | Paenibacillus thermoaerophilus |
Organism | Comment | Expression |
---|---|---|
Paenibacillus thermoaerophilus | adding D-glucose, lactose, D-mannose, or sucrose to culture medium decreases the amount of the enzyme | down |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | at pH 7.0 and 60°C | Paenibacillus thermoaerophilus |