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Literature summary for 3.2.1.14 extracted from

  • Niu, X.; Zhou, J.S.; Wang, Y.X.; Liu, C.C.; Liu, Z.H.; Yuan, S.
    Heterologous expression and characterization of a novel chitinase (ChiEn1) from Coprinopsis cinerea and its synergism in the degradation of chitin (2017), J. Agric. Food Chem., 65, 6943-6956 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Coprinopsis cinerea

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 9.6% residual activity at 1 mM Coprinopsis cinerea
Cr6+ 80.9% residual activity at 1 mM Coprinopsis cinerea
Cu2+ 28.5% residual activity at 1 mM Coprinopsis cinerea
EDTA 79.7% residual activity at 1 mM, complete inhibition at 2 mM Coprinopsis cinerea
Fe3+ 27.3% residual activity at 1 mM Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.254
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 5.0 and 37°C Coprinopsis cinerea
2.342
-
 4-nitrophenyl N,N',N''-beta-D-triacetylchitotrioside at pH 5.0 and 37°C Coprinopsis cinerea

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 106% activity at 1 mM Coprinopsis cinerea
Ca2+ 123% activity at 1 mM Coprinopsis cinerea
Co2+ 170% activity at 1 mM Coprinopsis cinerea
Mg2+ 106% activity at 1 mM Coprinopsis cinerea
Mn2+ 295% activity at 1 mM Coprinopsis cinerea
Mo6+ 117% activity at 1 mM Coprinopsis cinerea
Ni2+ 126% activity at 1 mM Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-
Coprinopsis cinerea ATCC 56838
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Coprinopsis cinerea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N,N',N''-beta-D-triacetylchitotrioside + H2O
-
 Coprinopsis cinerea 4-nitrophenol + N,N',N''-beta-D-triacetylchitotriose + N,N'-diacetyl-beta-D-chitobiose + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
 ?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Coprinopsis cinerea 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
 ?
chitohexaose + H2O
-
 Coprinopsis cinerea ?
-
 ?
chitohexaose + H2O
-
 Coprinopsis cinerea ATCC 56838 ?
-
 ?
chitopentaose + H2O
-
 Coprinopsis cinerea ?
-
 ?
chitopentaose + H2O
-
 Coprinopsis cinerea ATCC 56838 ?
-
 ?
chitosan + H2O 85% deacetylated chitosan Coprinopsis cinerea ?
-
 ?
chitosan + H2O 85% deacetylated chitosan Coprinopsis cinerea ATCC 56838 ?
-
 ?
chitotetraose + H2O
-
 Coprinopsis cinerea ?
-
 ?
chitotetraose + H2O
-
 Coprinopsis cinerea ATCC 56838 ?
-
 ?
chitotriose + H2O
-
 Coprinopsis cinerea ?
-
 ?
additional information the enzyme ChiEn1 does not hydrolyze 4-nitrophenyl N-acetyl-beta-D-glucosaminide, carboxymethyl cellulose, laminarin, chitobiose, chitohexaose, insoluble chitin powder, colloidal chitin, and soluble glycol chitosan Coprinopsis cinerea ?
-
 ?
additional information the enzyme ChiEn1 does not hydrolyze 4-nitrophenyl N-acetyl-beta-D-glucosaminide, carboxymethyl cellulose, laminarin, chitobiose, chitohexaose, insoluble chitin powder, colloidal chitin, and soluble glycol chitosan Coprinopsis cinerea ATCC 56838 ?
-
 ?
soluble glycol chitin + H2O
-
 Coprinopsis cinerea ?
-
 ?

Synonyms

Synonyms Comment Organism
ChiEN1
-
 Coprinopsis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 at pH 5.0 Coprinopsis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 enzyme activity decreases with increasing incubation temperature after being incubated for 30 min at 20-70°C Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13
-
 4-nitrophenyl N,N',N''-beta-D-triacetylchitotrioside at pH 5.0 and 37°C Coprinopsis cinerea
2.28
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 5.0 and 37°C Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 at 37°C Coprinopsis cinerea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 after incubation of the enzyme over a broad range of pH (4.0-9.0) for 30 min, its hydrolytic activity does not significantly change Coprinopsis cinerea

Expression

Organism Comment Expression
Coprinopsis cinerea the enzyme gene expression increases with the maturation of fruiting bodies up

General Information

General Information Comment Organism
metabolism the enzyme ChiEn1 acts synergistically with the endochitinase ChiIII to efficiently hydrolyze insoluble chitin polymers Coprinopsis cinerea