Cloned (Comment) | Organism |
---|---|
a C-terminal truncated form of chiA lacking a putative chitin-binding domain, recombinant expression of the N-terminally His-tagged mutant in Escherichia coli strain BL21 (DE3) | Salmonella enterica typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71365 | - |
x * 71365, truncated ChiA mutant enzyme ChiADELTACtBM, sequence calculation | Salmonella enterica typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica typhimurium | - |
gene SL0018 or chiA | - |
Salmonella enterica typhimurium SL1344 | - |
gene SL0018 or chiA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Salmonella enterica typhimurium | ? | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Salmonella enterica typhimurium SL1344 | ? | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Salmonella enterica typhimurium | ? | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Salmonella enterica typhimurium SL1344 | ? | - |
? | |
carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O | - |
Salmonella enterica typhimurium | ? | - |
? | |
carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O | - |
Salmonella enterica typhimurium SL1344 | ? | - |
? | |
chitin + H2O | - |
Salmonella enterica typhimurium | N,N'-diacetylchitobiose + ? | - |
? | |
chitin + H2O | - |
Salmonella enterica typhimurium SL1344 | N,N'-diacetylchitobiose + ? | - |
? | |
additional information | the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids | Salmonella enterica typhimurium | ? | - |
? | |
additional information | the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids | Salmonella enterica typhimurium SL1344 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71365, truncated ChiA mutant enzyme ChiADELTACtBM, sequence calculation | Salmonella enterica typhimurium |
Synonyms | Comment | Organism |
---|---|---|
ChiA | - |
Salmonella enterica typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Salmonella enterica typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Salmonella enterica typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | high activity in this range | Salmonella enterica typhimurium |