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Literature summary for 3.2.1.14 extracted from

  • Vincenzi, S.; Bierma, J.; Wickramasekara, S.I.; Curioni, A.; Gazzola, D.; Bakalinsky, A.T.
    Characterization of a grape class IV chitinase (2014), J. Agric. Food Chem., 62, 5660-5668.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene VvChi4D, DNA and amino acid sequence determination and analysis Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Vitis vinifera chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine N,N'-diacetylchitobiose + ?
-
 ?
chitin + H2O Vitis vinifera Manzoni Bianco chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine N,N'-diacetylchitobiose + ?
-
 ?
N-acetylglucosamine oligomers + H2O Vitis vinifera
-
 N-acetylglucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Vitis vinifera Q7XAU6 gene VvChi4D
-
Vitis vinifera Manzoni Bianco Q7XAU6 gene VvChi4D
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from filtered grape juice in a procedure including cation exchange chromatography and dialysis Vitis vinifera

Source Tissue

Source Tissue Comment Organism Textmining
fruit grape juice, type IV grape chitinase Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O crab shell chitin Vitis vinifera ?
-
 ?
chitin + H2O crab shell chitin Vitis vinifera Manzoni Bianco ?
-
 ?
chitin + H2O chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine Vitis vinifera N,N'-diacetylchitobiose + ?
-
 ?
chitin + H2O chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine Vitis vinifera Manzoni Bianco N,N'-diacetylchitobiose + ?
-
 ?
chitohexaose + H2O reaction via trimer formation Vitis vinifera 2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose
-
 ?
chitopentaose + H2O reaction via trimer formation Vitis vinifera N-acetylglucosamine + 2 N,N'-diacetylchitobiose
-
 ?
colloidal chitin + H2O
-
 Vitis vinifera N,N'-diacetylchitobiose + ? the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity ?
colloidal chitin + H2O
-
 Vitis vinifera Manzoni Bianco N,N'-diacetylchitobiose + ? the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity ?
additional information type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity Vitis vinifera ?
-
 ?
additional information type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity Vitis vinifera Manzoni Bianco ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O reaction via trimer formation Vitis vinifera 2 N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Vitis vinifera N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Vitis vinifera Manzoni Bianco N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N-acetylglucosamine oligomers + H2O
-
 Vitis vinifera N-acetylglucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Vitis vinifera
More tryptic peptide mapping and mass spectrometric analysis Vitis vinifera

Synonyms

Synonyms Comment Organism
class IV chitinase
-
 Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vitis vinifera

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, at above 50°C, activity decreases drastically, profile overview Vitis vinifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 Tm at pH 4.0 Vitis vinifera
70
-
 purified enzyme, pH 6.0, 1 h, 30% activity remaining Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Vitis vinifera

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 narrow pH spectrum, sharp decrease in activity below and above Vitis vinifera

General Information

General Information Comment Organism
evolution the enzyme belongs to carbohydrate-binding module family 18 and glycoside hydrolase family GH19. Possibly the two proteins class IV chitinase and class IV endochitinase, UniProt IDs Q7XAU6 and O24530, are allelic variants encoded by the same gene Vitis vinifera
additional information at a concentration and pH expected in ripe grapes, no inhibition of wine yeast growth by the chitinase is observed Vitis vinifera