Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | 118% activity at 2 mM | Hydrogenophilus hirschii | |
1-Hexanol | about 140% activity at 25% (v/v) | Hydrogenophilus hirschii | |
ethanol | about 127% activity at 25% (v/v) | Hydrogenophilus hirschii | |
methanol | about 125% activity at 25% (v/v) | Hydrogenophilus hirschii | |
n-heptane | about 120% activity at 25% (v/v) | Hydrogenophilus hirschii | |
n-hexane | about 130% activity at 25% (v/v) | Hydrogenophilus hirschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | about 80% residual activity at 25% (v/v) | Hydrogenophilus hirschii | |
2-mercaptoethanol | about 10% residual activity at 5 mM | Hydrogenophilus hirschii | |
4-chloromercuribenzoic acid | complete inhibition at 2 mM | Hydrogenophilus hirschii | |
5,5'-dithiobis-(2-nitrobenzoic acid) | about 8% residual activity at 2 mM | Hydrogenophilus hirschii | |
acetonitrile | complete inhibition at 25% (v/v) | Hydrogenophilus hirschii | |
Ag+ | 88% inhibition at 2 mM | Hydrogenophilus hirschii | |
Al3+ | 39% inhibition at 2 mM | Hydrogenophilus hirschii | |
Ba2+ | 24% inhibition at 2 mM | Hydrogenophilus hirschii | |
Cd2+ | complete inhibition at 2 mM | Hydrogenophilus hirschii | |
dithiothreitol | about 5% residual activity at 1 mM | Hydrogenophilus hirschii | |
ethyl acetate | complete inhibition at 25% (v/v) | Hydrogenophilus hirschii | |
Fe2+ | 55% inhibition at 2 mM | Hydrogenophilus hirschii | |
Hg2+ | complete inhibition at 2 mM | Hydrogenophilus hirschii | |
isooctane | about 80% residual activity at 25% (v/v) | Hydrogenophilus hirschii | |
additional information | not significantly inhibited by 2,4,6-trinitrobenzenesulfonic acid, phenylmethylsulfonyl fluoride, diethyldicarbonate, N-bromosuccinimide, and N-acetylimidazole | Hydrogenophilus hirschii | |
N-ethylmaleimide | complete inhibition at 2 mM | Hydrogenophilus hirschii | |
Ni2+ | complete inhibition at 2 mM | Hydrogenophilus hirschii | |
Zn2+ | 10% inhibition at 2 mM | Hydrogenophilus hirschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 130% activity at 2 mM | Hydrogenophilus hirschii | |
Co2+ | 105% activity at 2 mM | Hydrogenophilus hirschii | |
Cu2+ | 111% activity at 2 mM | Hydrogenophilus hirschii | |
Mg2+ | 305% activity at 2 mM | Hydrogenophilus hirschii | |
Mn2+ | 197% activity at 2 mM | Hydrogenophilus hirschii | |
additional information | K+ and Li+ do not show significant inhibition or activation effects | Hydrogenophilus hirschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenophilus hirschii | - |
- |
- |
Hydrogenophilus hirschii KB-DZ44 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration and Mono Q Sepharose column chromatography | Hydrogenophilus hirschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O | 700% activity compared to colloidal chitin | Hydrogenophilus hirschii | 4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O | 250% activity compared to colloidal chitin | Hydrogenophilus hirschii | 4-nitrophenyl beta-D-N,N'-diacetylchitobioside | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | 185% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
chitin azure + H2O | 98% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
chitin azure + H2O | 98% activity compared to colloidal chitin | Hydrogenophilus hirschii KB-DZ44 | ? | - |
? | |
chitooligosaccharide + H2O | 76% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
chitotriose + H2O | 85% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
colloidal chitin + H2O | 100% activity | Hydrogenophilus hirschii | ? | - |
? | |
glycol chitin + H2O | 95% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
glycol chitin + H2O | 95% activity compared to colloidal chitin | Hydrogenophilus hirschii KB-DZ44 | ? | - |
? | |
glycol chitosan + H2O | 90% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
glycol chitosan + H2O | 90% activity compared to colloidal chitin | Hydrogenophilus hirschii KB-DZ44 | ? | - |
? | |
additional information | the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose | Hydrogenophilus hirschii | ? | - |
? | |
additional information | the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose | Hydrogenophilus hirschii KB-DZ44 | ? | - |
? | |
xylan + H2O | 46% activity compared to colloidal chitin | Hydrogenophilus hirschii | ? | - |
? | |
xylan + H2O | 46% activity compared to colloidal chitin | Hydrogenophilus hirschii KB-DZ44 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE | Hydrogenophilus hirschii |
monomer | 1 * 59103, MALDI-TOF mass spectrometry | Hydrogenophilus hirschii |
Synonyms | Comment | Organism |
---|---|---|
ChiA-Hh59 | - |
Hydrogenophilus hirschii |
endo-chitinase | - |
Hydrogenophilus hirschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Hydrogenophilus hirschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | the half-life times at 70, 80, 90, and 100°C in the absence of Mg2+ are 8, 5, 3, and 1 h, respectively. In the presence of 2 mM Mg2+, the half-life times of the enzyme increase to 10, 7, 4, and 2 h, respectively | Hydrogenophilus hirschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14400 | - |
colloidal chitin | at pH 5.0 and 70°C | Hydrogenophilus hirschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Hydrogenophilus hirschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the relative activities at pH 3.0 and 9.0 are 80 and 45%, respectively | Hydrogenophilus hirschii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | the half-life times of the enzyme at pH 3.0, 4.0, and 5.0 are 10, 8, and 6 h, respectively | Hydrogenophilus hirschii |