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Literature summary for 3.2.1.14 extracted from

  • Mizuno, R.; Fukamizo, T.; Sugiyama, S.; Nishizawa, Y.; Kezuka, Y.; Nonaka, T.; Suzuki, K.; Watanabe, T.
    Role of the loop structure of the catalytic domain in rice class I chitinase (2008), J. Biochem., 143, 487-495.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type OsChia1b and the two mutant chitinases, loop II-deletion mutant (OsChia1bDELTAloopII) and W159A mutant (OsChia1bW159A) in Escherichia coli Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information the loop II deletion and the W159A mutation increases hydrolytic activity toward N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose and toward polysaccharide substrates. Similar results are obtained for kcat/Km values determined for substrate reduced N-acetylchitopentaose. The two mutations shift the splitting positions in N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to the reducing end side, but the shift is less intensive in the Trp mutant Oryza sativa
W159A mutation increases hydrolytic activity toward N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose and toward polysaccharide substrates. Similar results are obtained for kcat/Km value determined for substrate reduced N-acetylchitopentaose. The mutation shifts the splitting positions in N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to the reducing end side Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
reduced N-acetylchitopentaose wild-type enzyme Oryza sativa
0.69
-
reduced N-acetylchitopentaose mutant W159A Oryza sativa
0.72
-
reduced N-acetylchitopentaose DELTAloop II mutant Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
191
-
wild-type enzyme, substrate: colloidal chitin Oryza sativa
232
-
wild-type enzyme, substrate: glycol chitin Oryza sativa
265
-
DELTAloop II enzyme, substrate: colloidal chitin Oryza sativa
399
-
mutant enzyme W159A, substrate: colloidal chitin Oryza sativa
479
-
DELTAloop II enzyme, substrate: glycol chitin Oryza sativa
946
-
mutant enzyme W159A, substrate: glycol chitin Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O wild-type enzyme: the alpha/beta ratios of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose are 26.8 and 2.95, respectively Oryza sativa N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
?
reduced N-acetylchitopentaose + H2O
-
Oryza sativa ?
-
?

Synonyms

Synonyms Comment Organism
OsChia1b
-
Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.32
-
reduced N-acetylchitopentaose wild-type enzyme Oryza sativa
10.6
-
reduced N-acetylchitopentaose DELTAloop II mutant Oryza sativa
20.9
-
reduced N-acetylchitopentaose mutant W159A Oryza sativa