Cloned (Comment) | Organism |
---|---|
expression of wild-type OsChia1b and the two mutant chitinases, loop II-deletion mutant (OsChia1bDELTAloopII) and W159A mutant (OsChia1bW159A) in Escherichia coli | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the loop II deletion and the W159A mutation increases hydrolytic activity toward N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose and toward polysaccharide substrates. Similar results are obtained for kcat/Km values determined for substrate reduced N-acetylchitopentaose. The two mutations shift the splitting positions in N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to the reducing end side, but the shift is less intensive in the Trp mutant | Oryza sativa |
W159A | mutation increases hydrolytic activity toward N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose and toward polysaccharide substrates. Similar results are obtained for kcat/Km value determined for substrate reduced N-acetylchitopentaose. The mutation shifts the splitting positions in N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to the reducing end side | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
reduced N-acetylchitopentaose | wild-type enzyme | Oryza sativa | |
0.69 | - |
reduced N-acetylchitopentaose | mutant W159A | Oryza sativa | |
0.72 | - |
reduced N-acetylchitopentaose | DELTAloop II mutant | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
191 | - |
wild-type enzyme, substrate: colloidal chitin | Oryza sativa |
232 | - |
wild-type enzyme, substrate: glycol chitin | Oryza sativa |
265 | - |
DELTAloop II enzyme, substrate: colloidal chitin | Oryza sativa |
399 | - |
mutant enzyme W159A, substrate: colloidal chitin | Oryza sativa |
479 | - |
DELTAloop II enzyme, substrate: glycol chitin | Oryza sativa |
946 | - |
mutant enzyme W159A, substrate: glycol chitin | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | wild-type enzyme: the alpha/beta ratios of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose are 26.8 and 2.95, respectively | Oryza sativa | N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
reduced N-acetylchitopentaose + H2O | - |
Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OsChia1b | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.32 | - |
reduced N-acetylchitopentaose | wild-type enzyme | Oryza sativa | |
10.6 | - |
reduced N-acetylchitopentaose | DELTAloop II mutant | Oryza sativa | |
20.9 | - |
reduced N-acetylchitopentaose | mutant W159A | Oryza sativa |