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Literature summary for 3.2.1.14 extracted from

  • Mizuno, R.; Itoh, Y.; Nishizawa, Y.; Kezuka, Y.; Suzuki, K.; Nonaka, T.; Watanabe, T.
    Purification and characterization of a rice class I chitinase, OsChia1b, produced in Escherichia coli (2008), Biosci. Biotechnol. Biochem., 72, 893-895.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene Cht-2, expression in Escherichia coli Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Oryza sativa chitinase is a glycoside hydrolase that catalyzes the degradation of chitin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 ssp. japonica, cv. Nipponbare, gene Cht-2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant OsChia1b from Escherichia coli by ammonium sulfate fractionation and chitin affinity chromatography to homogeneity Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
225
-
 purified OsChia1b, substrate colloidal chitin Oryza sativa
253
-
 purified OsChia1b, substrate glycol chitin Oryza sativa
254
-
 purified OsChia1b, substrate GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc Oryza sativa
2211
-
 purified OsChia1b, substrate WS-chitin Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O chitinase is a glycoside hydrolase that catalyzes the degradation of chitin Oryza sativa ?
-
 ?
chitin + H2O WS-chitin and colloidal chitin substrate Oryza sativa ?
-
 ?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Oryza sativa ?
-
 ?
glycol chitin + H2O
-
 Oryza sativa ?
-
 ?
additional information OsChia1b shows no activity against N-acetylchitotriose, and crystalline beta-chitin is a poor substrate. OsChia1b substrate binding specificity depends on the N-acetyl groups of the substrate. About 80% of added OsChia1b binds to colloidal chitin, whereas less than 30% of added OsChia1b binds to chitosan 7B, with 70% deacetylated chitin, avicel, and acid-swollen cellulose, less than 10% of OsChia1b binds to beta-1,3-glucan, curdlan Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
class I chitinase
-
 Oryza sativa
More OsChia1b is a class I chitinase of glucohydrolase family 19 Oryza sativa
OsChia1b
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80
-
 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
2 12
-
 Oryza sativa