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Literature summary for 3.2.1.14 extracted from

  • Liu, C.L.; Shen, C.R.; Hsu, F.F.; Chen, J.K.; Wu, P.T.; Guo, S.H.; Lee, W.C.; Yu, F.W.; Mackey, Z.B.; Turk, J.; Gross, M.L.
    Isolation and identification of two novel SDS-resistant secreted chitinases from Aeromonas schubertii (2009), Biotechnol. Prog., 25, 124-131.
    View publication on PubMedView publication on EuropePMC
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General Stability

General Stability Organism
ASCHI53 and ASCHI61 retain, respectively, 65% and 75%, of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% 2-mercaptoethanol Aeromonas schubertii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53527
-
 x * 53527, isoform ASCHI53, x * 61202, isoform ASCHI61, MALDI-TOF. x * 4300, isoform ASCHI53, x * 55000, isoform ASCHI61, SDS-PAGE Aeromonas schubertii
55000
-
 x * 53527, isoform ASCHI53, x * 61202, isoform ASCHI61, MALDI-TOF. x * 4300, isoform ASCHI53, x * 55000, isoform ASCHI61, SDS-PAGE Aeromonas schubertii
61202
-
 x * 53527, isoform ASCHI53, x * 61202, isoform ASCHI61, MALDI-TOF. x * 4300, isoform ASCHI53, x * 55000, isoform ASCHI61, SDS-PAGE Aeromonas schubertii

Organism

Organism UniProt Comment Textmining
Aeromonas schubertii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas schubertii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Aeromonas schubertii ? formation of hexameric chitooligosaccharide ?

Subunits

Subunits Comment Organism
? x * 53527, isoform ASCHI53, x * 61202, isoform ASCHI61, MALDI-TOF. x * 4300, isoform ASCHI53, x * 55000, isoform ASCHI61, SDS-PAGE Aeromonas schubertii