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Literature summary for 3.2.1.14 extracted from

  • Cheng, X.X.; Zhao, L.H.; Klosterman, S.J.; Feng, H.J.; Feng, Z.L.; Wei, F.; Shi, Y.Q.; Li, Z.F.; Zhu, H.Q.
    The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses (2017), Plant Sci., 259, 12-23 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Verticillium dahliae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Verticillium dahliae
-
 ?
-
 ?
chitin + H2O Verticillium dahliae Vd080
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Verticillium dahliae A0A286LWZ1
-
-
Verticillium dahliae Vd080 A0A286LWZ1
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged purification Miniprep kit Verticillium dahliae

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Verticillium dahliae ?
-
 ?
chitin + H2O
-
 Verticillium dahliae Vd080 ?
-
 ?
chitohexaose + H2O
-
 Verticillium dahliae chitobiose + chitotetraose + chitotriose
-
 ?
chitohexaose + H2O
-
 Verticillium dahliae Vd080 chitobiose + chitotetraose + chitotriose
-
 ?

Subunits

Subunits Comment Organism
? x * 114000, His-tagged enzyme, SDS-PAGE Verticillium dahliae
? x * 62200, His-tagged enzyme, calculated from amino acid sequence Verticillium dahliae

Synonyms

Synonyms Comment Organism
ECH
-
 Verticillium dahliae
endochitinase
-
 Verticillium dahliae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Verticillium dahliae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 enzyme activity is retained after 15 min at 100°C Verticillium dahliae

General Information

General Information Comment Organism
physiological function the enzyme inhibits spore germination and activates plant defense responses Verticillium dahliae