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Literature summary for 3.2.1.14 extracted from
- Expression, purification and in vitro antifungal activity of acidic mammalian chitinase against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains (2009), Clin. Exp. Dermatol., 34, 55-60.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged AMCase in Escherichia coli | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 78000, recombinant GST-tagged AMCase, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview | ? | - |
? |  |
| additional information | Mus musculus BALB/c | AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
Balb/c mice | - |
| Mus musculus BALB/c | - |
Balb/c mice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from stomach and recombinant GST-tagged enzyme to homogeneity | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stomach | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
antifungal potency of native and recombinant enzymes, overview | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview | Mus musculus | ? | - |
? | |
| additional information | native AMCase from the BALB/c mouse stomach shows higher chitinolytic activity compared with the hydrolytic effects of the recombinant AMCase protein | Mus musculus | ? | - |
? | |
| additional information | AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview | Mus musculus BALB/c | ? | - |
? | |
| additional information | native AMCase from the BALB/c mouse stomach shows higher chitinolytic activity compared with the hydrolytic effects of the recombinant AMCase protein | Mus musculus BALB/c | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, recombinant GST-tagged AMCase, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic mammalian chitinase | - |
Mus musculus |
| AMCase | - |
Mus musculus |
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