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Literature summary for 3.2.1.14 extracted from

  • De Siqueira Pinto, A.; Barreto, C.C.; Schrank, A.; Ulhoa, C.J.; Henning Vainstein, M.
    Purification and characterization of an extracellular chitinase from the entomopathogen Metarhizium anisopliae (1997), Can. J. Microbiol., 43, 322-327.
No PubMed abstract available
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.537
-
 p-nitrophenyl beta-D-N,N'-diacetylchitobiose
-
 Metarhizium anisopliae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Metarhizium anisopliae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Metarhizium anisopliae
32400
-
 gel filtration Metarhizium anisopliae

Organism

Organism UniProt Comment Textmining
Metarhizium anisopliae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2.5fold Metarhizium anisopliae

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Metarhizium anisopliae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose Metarhizium anisopliae
1.16
-
 p-nitrophenyl beta-D-N,N'-diacetylchitobiose Metarhizium anisopliae
3.2
-
 swollen chitin Metarhizium anisopliae
3.6
-
 p-nitrophenyl N-acetyl-beat-D-glucosaminide Metarhizium anisopliae
6.74
-
 chitobiose Metarhizium anisopliae
8.57
-
 chitotriose Metarhizium anisopliae
10.48
-
 chitotetraose Metarhizium anisopliae
146
-
-
 Metarhizium anisopliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O swollen chitin Metarhizium anisopliae N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
chitobiose + H2O
-
 Metarhizium anisopliae 2 D-glucosamine
-
 ?
chitotetraose + H2O
-
 Metarhizium anisopliae N-acetylglucosamine + chitobiose + chitotriose N-acetylglucosamine only ?
N,N',N''-triacetylchitotriose + H2O
-
 Metarhizium anisopliae N-acetylglucosamine + N,N'-diacetylchitobiose N-acetylglucosamine only ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
-
 Metarhizium anisopliae ?
-
 ?
p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Metarhizium anisopliae ?
-
 ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Metarhizium anisopliae p-nitrophenol + N,N'-diacetylchitobiose
-
 ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Metarhizium anisopliae N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Metarhizium anisopliae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Metarhizium anisopliae