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Literature summary for 3.2.1.14 extracted from

  • Taira, T.; Hayashi, H.; Tajiri, Y.; Onaga, S.; Uechi, G.; Iwasaki, H.; Ohnuma, T.; Fukamizo, T.
    A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational modification (2009), Glycobiology, 19, 1452-1461.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cycas revoluta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE of mature protein Cycas revoluta

Organism

Organism UniProt Comment Textmining
Cycas revoluta Q4W6L6 isoform ChiA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein mature pürotein is N-glycosylated at residue N222 Cycas revoluta
proteolytic modification sequence contains a N-terminal signal peptide, mature protein comprises residues Q24-G370 Cycas revoluta

Purification (Commentary)

Purification (Comment) Organism
-
 Cycas revoluta

Source Tissue

Source Tissue Comment Organism Textmining
leaf leaf rachise Cycas revoluta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Cycas revoluta 2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc main product, minor products (GlcNAc)2 and (GlcNAc)4 are produced in the molar ratio (GlcNAc)3:(GlcNAc)2:(GlcNAc)4 of 1:0.3:0.4 ?
additional information enzyme exhibits transglycosylation activity Cycas revoluta ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE of mature protein Cycas revoluta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Cycas revoluta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Cycas revoluta

pI Value

Organism Comment pI Value Maximum pI Value
Cycas revoluta isoelectric focusing
-
 5.6