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Literature summary for 3.2.1.14 extracted from

  • Bokma, E.; Rozeboom, H.J.; Sibbald, M.; Dijkstra, B.W.; Beintema, J.J.
    Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis (2002), Eur. J. Biochem., 269, 893-901.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hevea brasiliensis

Crystallization (Commentary)

Crystallization (Comment) Organism
grown from 1.1-1.4 M ammonium sulfate or PEG3350 solutions, pH 7 Hevea brasiliensis

Protein Variants

Protein Variants Comment Organism
D125A nearly complete loss in activity Hevea brasiliensis
D125N twofold lower turnover and twofold higher KM than wild-type, also active at higher pH-values Hevea brasiliensis
E127A nearly complete loss in activity Hevea brasiliensis
Y183F lower turnover than wild-type Hevea brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, Y183F Hevea brasiliensis
0.016
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, D125N Hevea brasiliensis
0.02
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, recombinant wild-type Hevea brasiliensis
0.028
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, purified wild-type Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitopentaose + H2O
-
 Hevea brasiliensis ?
-
 ?
colloidal chitin + H2O
-
 Hevea brasiliensis ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
-
 Hevea brasiliensis 5 N-acetyl-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
hevamine
-
 Hevea brasiliensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, Y183F Hevea brasiliensis
0.28
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, D125N Hevea brasiliensis
0.61
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, recombinant wild-type Hevea brasiliensis
0.77
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose 30°C, pH 4.2, purified wild-type Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 with chitopentaose as substrate Hevea brasiliensis