Application | Comment | Organism |
---|---|---|
agriculture | the enzyme might be used in biotechnological strategies of insect management for stored cowpeas | Scorpaena scrofa |
General Stability | Organism |
---|---|
Co2+ stabilizes the purified enzyme | Scorpaena scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition | Scorpaena scrofa | |
Hg+ | complete inhibition | Scorpaena scrofa | |
Hg2+ | complete inhibition | Scorpaena scrofa | |
N-ethylmaleimide | complete inhibition | Scorpaena scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Scorpaena scrofa | |
0.419 | - |
colloidal chitin | pH 5.0, 37°C | Scorpaena scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Scorpaena scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | highl activating at 2 mM, Co2+ stabilizes the purified enzyme | Scorpaena scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Scorpaena scrofa |
50103 | - |
1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE | Scorpaena scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Scorpaena scrofa | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scorpaena scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 162.75fold from stomach to homogeneity by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Scorpaena scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Scorpaena scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.51 | - |
purified enzyme, pH 5.0, 37°C, substrate colloidal chitin | Scorpaena scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + 3 H2O | - |
Scorpaena scrofa | 4-nitrophenol + 2 N,N'-diacetylchitobiose | - |
? | |
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O | - |
Scorpaena scrofa | ? | - |
? | |
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O | - |
Scorpaena scrofa | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
chitin + H2O | - |
Scorpaena scrofa | ? | - |
? | |
chitotriose + H2O | - |
Scorpaena scrofa | ? | - |
? | |
colloidal chitin + H2O | - |
Scorpaena scrofa | ? | - |
? | |
glycol chitin + H2O | - |
Scorpaena scrofa | ? | - |
? | |
glycol chitosan + H2O | - |
Scorpaena scrofa | ? | - |
? | |
additional information | the enzyme hydrolyzes randomly beta-14,-N-acetylglucosamine linkages in the chitin polymer, and preferentially hydrolyzes the second glycosidic link from the nonreducing end of the N-acetylglucosamine oligomers | Scorpaena scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE | Scorpaena scrofa |
Synonyms | Comment | Organism |
---|---|---|
SsChi50 | - |
Scorpaena scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Scorpaena scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | over 50% activity in this range, profile overview | Scorpaena scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 10 h, half-life is 18 h, about 10% activity remaining after 24 h, without Co2+: completely stable for 6 h, half-life is 14 h, inactivation after 20 h | Scorpaena scrofa |
80 | - |
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 4 h, half-life is 12 h, inactivation after 16 h, without Co2+: completely stable for 2 h, half-life is 10 h, inactivation after 18 h | Scorpaena scrofa |
90 | - |
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 2 h, half-life is 8 h, inactivation after 12 h, without Co2+: half-life is 6 h, inactivation after 14 h | Scorpaena scrofa |
100 | - |
purified enzyme, pH 5.0, with 2 mM Co2+: half-life is 4 h, inactivation after 6 h, without Co2+: half-life is 2 h, inactivation after 8 h | Scorpaena scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.33 | - |
colloidal chitin | pH 5.0, 37°C | Scorpaena scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Scorpaena scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 11 | activity range, profile overview | Scorpaena scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, 60°C, 48 h, loss of 25% activity | Scorpaena scrofa |
3 | 7 | purified enzyme, 60°C, 48 h, completely stable at | Scorpaena scrofa |
8 | - |
purified enzyme, 60°C, 48 h, loss of 20% activity | Scorpaena scrofa |
9 | - |
purified enzyme, 60°C, 48 h, loss of 30% activity | Scorpaena scrofa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family GH18 | Scorpaena scrofa |
physiological function | the chitinase from the stomach of the red scorpionfish Scorpaena scrofa has bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus | Scorpaena scrofa |