KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-nitrophenyl N-acetyl-D-glucosaminide | at pH 5.0 and 40°C | Euphorbia characias |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 494% activity at 0.5 mM | Euphorbia characias | |
Mg2+ | 416% activity at 5 mM | Euphorbia characias | |
additional information | Cu2+, Mn2+, Ni2+, and Fe2+ do not influence the enzyme activity | Euphorbia characias |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euphorbia characias | U5LKV8 | - |
- |
Purification (Comment) | Organism |
---|---|
magnetic chitin affinity adsorbation and DEAE-cellulose column chromatography | Euphorbia characias |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Euphorbia characias | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-D-glucosaminide + H2O | - |
Euphorbia characias | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
chitobiose + H2O | - |
Euphorbia characias | ? | - |
? | |
chitotriose + H2O | - |
Euphorbia characias | ? | - |
? | |
colloidal chitin + H2O | - |
Euphorbia characias | N-acetyl-beta-D-glucosamine + chitobiose + chitotriose | - |
? | |
N-acetyl-beta-D-glucosamine + H2O | - |
Euphorbia characias | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36500, SDS-PAGE | Euphorbia characias |
Synonyms | Comment | Organism |
---|---|---|
chitinase III | - |
Euphorbia characias |
ELC | - |
Euphorbia characias |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Euphorbia characias |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | more than 50% activity between 37 and 55°C | Euphorbia characias |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the enzyme remains stable (more than 90% activity) after 10 min at 40-70°C. After 20 min at 40-50°C, the enzyme retains more than 90% activity, while at 60°C the enzyme activity drops to 50%. The enzyme is inactive after 30 min at 70°C | Euphorbia characias |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
4-nitrophenyl N-acetyl-D-glucosaminide | at pH 5.0 and 40°C | Euphorbia characias |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Euphorbia characias |