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Literature summary for 3.2.1.14 extracted from
- Cloning and characterization of a chitinase from Thermobifida fusca reveals Tfu_0580 as a thermostable and acidic endochitinase (2018), Biotechnol. Rep., 19, e00274 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermobifida fusca |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 125% activity at 1 mM | Thermobifida fusca |  |
| Mn2+ | 119% activity at 1 mM | Thermobifida fusca | |
| additional information | not influenced by K+, Na+, Mg2+, and Zn2+ | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | Q47SE9 | - |
- |
| Thermobifida fusca ATCC BAA-629 | Q47SE9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-charged his-affinity gel chromatography | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Thermobifida fusca | ? | - |
? | |
| 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
| 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Thermobifida fusca | ? | - |
? | |
| 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
| colloidal chitin + H2O | low activity | Thermobifida fusca | ? | - |
? | |
| colloidal chitin + H2O | low activity | Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
| additional information | the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Thermobifida fusca | ? | - |
? | |
| additional information | the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44900, SDS-PAGE | Thermobifida fusca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endochitinase | - |
Thermobifida fusca |
| Tfu_0580 | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Thermobifida fusca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | the enzyme retains more than 80% activity between 20 and 55°C, while the chitinase activity drops to less than 40% when temperature is above 65°C | Thermobifida fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermobifida fusca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 7.5 | the enzyme tends to be active under an acidic condition, which remain more than 60% highest activity between pH 4.8 and 7.5. When pH is at 8.0, the enzyme activity drops to 40% of its highest activity | Thermobifida fusca |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermobifida fusca | calculated from amino acid sequence | - |
5.8 |
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