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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of Bombyx mori chitinases (1997), Insect Biochem. Mol. Biol., 27, 757-767.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chitopentaose | - |
Bombyx mori |  |
| CuSO4 | isoenzyme 1 | Bombyx mori | |
| HgCl2 | both isoenzymes | Bombyx mori | |
| tetraacetylchitotetraose | - |
Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | isoenzyme 1 glycol chitin 0.134 mg/ml, isoenzyme 2 glycol chitin 0.023 mg/ml | Bombyx mori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2 | Bombyx mori |
| 88000 | - |
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2 | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 65 and 88 kDa isoenzymes | Bombyx mori |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme 1, 558fold to homogeneity, isoenzyme 2, 262fold to homogeneity | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | fifth-instar larvae integument | Bombyx mori | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitohexaose + H2O | - |
Bombyx mori | chitobiose + chitotriose | and chitotetraose | ? | |
| chitopentaose + H2O | - |
Bombyx mori | chitobiose + chitotriose | - |
? | |
| chitotetraose + H2O | - |
Bombyx mori | N-acetylglucosamine + chitobiose + chitotriose | chitobiose only | ? | |
| glycol chitin + H2O | - |
Bombyx mori | N-acetylglucosamine + ? | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | - |
Bombyx mori | N-acetylglucosamine + N,N'-diacetylchitobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2 | Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
both isoenzymes | Bombyx mori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below 30°C, isoenzyme 1 | Bombyx mori |
| 40 | - |
stable below 40°C | Bombyx mori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.059 | - |
glycol chitin | isoenzyme 2 | Bombyx mori | |
| 0.084 | - |
glycol chitin | isoenzyme 1 | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
N-acetylchitopentaose, isoenzyme 1 | Bombyx mori |
| 6.5 | - |
N-acetylchitopentaose, isoenzyme 2 | Bombyx mori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | both isoenzymes | Bombyx mori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | isoenzyme 1 | Bombyx mori |
| 6 | 12 | isoenzyme 2 | Bombyx mori |
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