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Literature summary for 3.2.1.14 extracted from
- Recombinant exochitinase of the thermophilic mould Myceliophthora thermophila BJA Characteristics and utility in generating N-acetyl glucosamine and in biocontrol of phytopathogenic fungi (2017), Biotechnol. Prog., 33, 70-80 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermothelomyces thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | 5 mM, 7% residual activity | Thermothelomyces thermophilus |  |
| Ag+ | 5 mM, 66% residual activity | Thermothelomyces thermophilus | |
| Cd2+ | 5 mM, 69% residual activity | Thermothelomyces thermophilus | |
| Hg2+ | 5 mM, 60% residual activity | Thermothelomyces thermophilus | |
| iodoacetamide | 5 mM, 51% residual activity | Thermothelomyces thermophilus | |
| Ni2+ | 5 mM, 72% residual activity | Thermothelomyces thermophilus | |
| Tween-80 | 10%, 49% residual activity | Thermothelomyces thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
colloidal chitin | Km value 4.6 mg/ml, pH 5, 55°C | Thermothelomyces thermophilus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 10%, 83% residual activity | Thermothelomyces thermophilus |
| benzene | 10%, 70% residual activity | Thermothelomyces thermophilus |
| Ethanol | 10%, 81% residual activity | Thermothelomyces thermophilus |
| Glycerol | 10%, 84% residual activity | Thermothelomyces thermophilus |
| hexane | 10%, 70% residual activity | Thermothelomyces thermophilus |
| isobutanol | 10%, 83% residual activity | Thermothelomyces thermophilus |
| toluene | 10%, 76% residual activity | Thermothelomyces thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | - |
- |
- |
| Thermothelomyces thermophilus BJA | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + H2O | - |
Thermothelomyces thermophilus | N-acetyl-D-glucosamine + ? | - |
? | |
| colloidal chitin + H2O | - |
Thermothelomyces thermophilus BJA | N-acetyl-D-glucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Chit | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
half-life 113 min | Thermothelomyces thermophilus |
| 75 | - |
half-life 48 min | Thermothelomyces thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermothelomyces thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
3 h, 84% residual activity | Thermothelomyces thermophilus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme inhibits the growth of phytopathogenic fungi like Fusarium oxysporum and Curvularia lunata | Thermothelomyces thermophilus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
colloidal chitin | Km value 4.6 mg/ml, pH 5, 55°C | Thermothelomyces thermophilus |
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Release 2023.1 (January 2023)
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