Activating Compound | Comment | Organism | Structure |
---|---|---|---|
LlCBP33A | a family 33 chitin-binding protein, binds to both alpha- and beta-chitin. LlCBP33A increases the hydrolytic efficiency of chitinase LlChi18A to both alpha- and beta-chitin | Lactococcus lactis |
Cloned (Comment) | Organism |
---|---|
gene LlChi18A, DNA and amino acid sequence determination and analysis, expression of the soluble His-tagged enzyme in Escherichia coli BL21(DE3) | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-chitin + H2O | Lactococcus lactis | - |
? | - |
? | |
beta-chitin + H2O | Lactococcus lactis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q9CE95 | ssp. lactis, strain IL1403, a derivative of the strain IL594 that is isolated from a cheese starter culture, gene LlChi18A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LlChi18A from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cleavage of the tag through factor Xa to a purity of over 95% | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O | - |
Lactococcus lactis | 4-methylumbelliferone + N,N'-diacetylchitobiose | - |
? | |
alpha-chitin + H2O | - |
Lactococcus lactis | ? | - |
? | |
beta-chitin + H2O | - |
Lactococcus lactis | ? | - |
? | |
additional information | LlChi18A has a shallow substrate-binding groove characteristic of nonprocessive endochitinases. LlChi18A is able to hydrolyse both chitin oligomers and artificial substrates, with no sign of processivity. Substrate specificity and binding preferences, overview | Lactococcus lactis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LlChi18A | - |
Lactococcus lactis |
More | th enzyme belongs to the family 18 of glycohydrolases | Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | - |
- |
Lactococcus lactis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.4 | - |
purified recombinant enzyme, stable | Lactococcus lactis |
5 | - |
and above, purified recombinant enzyme, stable for at least 1 week | Lactococcus lactis |