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Literature summary for 3.2.1.14 extracted from

  • Busswinkel, F.; Goni, O.; Cord-Landwehr, S.; OConnell, S.; Moerschbacher, B.M.
    Endochitinase 1 (Tv-ECH1) from Trichoderma virens has high subsite specificities for acetylated units when acting on chitosans (2018), Int. J. Biol. Macromol., 114, 453-461 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Application

Application Comment Organism
biotechnology the enzyme is used for the biotechnological production of specific chitosan oligomers and for the characterization of chitosan polymers via enzymatic fingerprinting Trichoderma virens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris PichiaPink Strain 1 Trichoderma virens

Organism

Organism UniProt Comment Textmining
Trichoderma virens Q8TGW8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Trichoderma virens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl chitotrioside + H2O
-
 Trichoderma virens ?
-
 ?
beta-D-N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Trichoderma virens beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Trichoderma virens beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Trichoderma virens 2 beta-D-N,N'-diacetylchitobiose
-
 ?
colloidal chitin + H2O
-
 Trichoderma virens ?
-
 ?
additional information the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose, beta-D-N,N',N''-triacetylchitotriose, alpha-chitin and beta-chitin Trichoderma virens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Trichoderma virens
? x * 45300, calculated from amino acid sequence Trichoderma virens

Synonyms

Synonyms Comment Organism
ECH1
-
 Trichoderma virens
endochitinase 1
-
 Trichoderma virens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Trichoderma virens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 more than 50% activity between 20 and 50°C Trichoderma virens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the enzyme remains stable for at least 50 h at pH 4.5 and 37°C Trichoderma virens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.08
-
 colloidal chitin at pH 4.5 and 37°C Trichoderma virens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Trichoderma virens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 more than 90% activity between pH 4.5 and 6.0 Trichoderma virens