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Literature summary for 3.2.1.14 extracted from

  • Goni, O.; Sanchez-Ballesta, M.T.; Merodio, C.; Escribano, M.I.
    Potent cryoprotective activity of cold and CO2-regulated cherimoya (Annona cherimola) endochitinase (2010), J. Plant Physiol., 167, 1119-1129.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics Annona cherimola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14309
-
 1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry Annona cherimola
14310
-
 1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry Annona cherimola
14500
-
 native PAGE Annona cherimola

Organism

Organism UniProt Comment Textmining
Annona cherimola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 36.2fold from the mesocarp of fruits by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatography and chromatofocusing Annona cherimola

Source Tissue

Source Tissue Comment Organism Textmining
fruit mesocarp of CO2-treated cherimoya fruit Annona cherimola
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3458
-
 purified enzyme, pH 5.0, 37°C Annona cherimola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Annona cherimola ?
-
 ?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Annona cherimola ?
-
 ?
4-methylumbelliferyl chitobioside + H2O
-
 Annona cherimola 4-methylumbelliferone + chitobiose
-
 ?
CM-chitin-RBV + H2O i.e carboxymethyl-Chitin-Remazol-Brilliant-Violet, a colloidal substrate Annona cherimola ?
-
 ?
additional information no activity with 4-methylumbelliferyl N-acetylglucosamine Annona cherimola ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry Annona cherimola
More peptide mapping by MALDI-TOF mass spectroemtry Annona cherimola

Synonyms

Synonyms Comment Organism
BChi14
-
 Annona cherimola
endochitinase
-
 Annona cherimola
More the enzyme belongs to the family 19 of glycosyl hydrolases, GH19 Annona cherimola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Annona cherimola

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50 30% of maximal activity at 5°C, 50% at 50°C Annona cherimola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5
-
 purified enzyme, 30% activity remaining Annona cherimola
50
-
 purified enzyme, stable up to, rapid loss of activity above Annona cherimola
60
-
 purified enzyme, loss of 70% activity Annona cherimola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.87
-
 4-methylumbelliferyl chitobioside pH 5.0, 37°C Annona cherimola
6.93
-
 4-methylumbelliferyl chitotriose pH 5.0, 37°C Annona cherimola
366.7
-
 CM-chitin-RBV pH 5.0, 37°C Annona cherimola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Annona cherimola

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 the chitinase isolated from cherimoya mesocarp apparently displays moderate enzymatic activity over a broad pH range, in both acidic and alkaline conditions, and with a neutral optimal pH Annona cherimola
3 9.5 80% of maximal activity at pH 3.0 and pH 9.0, 50% at pH 9.5 Annona cherimola

pI Value

Organism Comment pI Value Maximum pI Value
Annona cherimola isoelectric focusing
-
 8.26

General Information

General Information Comment Organism
physiological function BChi14 is functionally implicated in the mechanisms underlying chilling tolerance activated by high CO2 concentrations. The purified endochitinase shows very strong cryoprotective activity against freeze-thaw inactivation of lactate dehydrogenase, but the enzyme has no effect on the inhibition of Botrytis cinerea hyphal growth and no antifreeze activity Annona cherimola