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Literature summary for 3.2.1.14 extracted from

  • Stefanidi, E.; Vorgias, C.E.
    Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme (2008), Extremophiles, 12, 541-552.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF Moritella marina

Cloned(Commentary)

Cloned (Comment) Organism
gene chi60, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Moritella marina

Inhibitors

Inhibitors Comment Organism Structure
allosamidin 50% inhibition at 0.0005 mM Moritella marina
additional information the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF Moritella marina
Urea 30% inhibition at 5 M Moritella marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.101
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 0°C, purified recombinant enzyme Moritella marina
0.37
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 10°C, purified recombinant enzyme Moritella marina
0.416
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 15°C, purified recombinant enzyme Moritella marina
0.5
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 5°C or 20°C, purified recombinant enzyme Moritella marina
0.555
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 28°C, purified recombinant enzyme Moritella marina

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by various bivalent cations Moritella marina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Moritella marina
60788
-
1 * 60788, Chi60, sequence calculation Moritella marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Moritella marina chitinases hydrolyse the beta-1,4-linkages in chitin ?
-
?

Organism

Organism UniProt Comment Textmining
Moritella marina B1VBB0 i.e. Vibrio marinus, gene chi60
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Chi60 25fold from Escherichia coli Moritella marina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
purified recombinant enzyme, substrate powdered chitosan Moritella marina
0.16
-
purified recombinant enzyme, substrate colloidal chitin Moritella marina
0.91
-
purified recombinant enzyme, substrate 4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose Moritella marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-1,4-N,N'-diacetyl-chitobiose + H2O
-
Moritella marina 4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose
-
?
4-nitrophenyl beta-1,4-N,N'-diacetylchitobiose + H2O
-
Moritella marina ?
-
?
chitin + H2O chitinases hydrolyse the beta-1,4-linkages in chitin Moritella marina ?
-
?
colloidal chitin + H2O
-
Moritella marina ?
-
?
additional information the enzyme is not active with synthetic 4-nitrophenyl-linked glycosidic substrates, overview Moritella marina ?
-
?
powdered chitosan + H2O
-
Moritella marina ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60788, Chi60, sequence calculation Moritella marina
More Chi60 domain structure, overview Moritella marina

Synonyms

Synonyms Comment Organism
MmChi60
-
Moritella marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Moritella marina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
60
-
Moritella marina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme stability at 18°C and 28°C is similar to the stability at 4°C Moritella marina
-
30 purified recombinant enzyme, 72 h, completely stable Moritella marina
40
-
purified recombinant enzyme, half-life is 72 h Moritella marina
50
-
purified recombinant enzyme, half-life is 5 h Moritella marina
60
-
purified recombinant enzyme, half-life is 10 min Moritella marina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.79
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 0°C, purified recombinant enzyme Moritella marina
4.33
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 5°C, purified recombinant enzyme Moritella marina
5.46
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 10°C, purified recombinant enzyme Moritella marina
7.33
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 15°C, purified recombinant enzyme Moritella marina
11.88
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 20°C, purified recombinant enzyme Moritella marina
19.54
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 28°C, purified recombinant enzyme Moritella marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Moritella marina

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
Moritella marina

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
purified recombinant enzyme, 3 days, loss of 80% of activity at 4°C Moritella marina
4 10 the purified recombinant Chi60 exhibits broad pH stability maintaining over 60% activity after 10 days at a pH range at 4°C Moritella marina
8 10 purified recombinant enzyme, 10 days, no loss of activity at 4°C Moritella marina