Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF | Moritella marina |
Cloned (Comment) | Organism |
---|---|
gene chi60, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Moritella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
allosamidin | 50% inhibition at 0.0005 mM | Moritella marina | |
additional information | the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF | Moritella marina | |
Urea | 30% inhibition at 5 M | Moritella marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.101 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 0°C, purified recombinant enzyme | Moritella marina | |
0.37 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 10°C, purified recombinant enzyme | Moritella marina | |
0.416 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 15°C, purified recombinant enzyme | Moritella marina | |
0.5 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 5°C or 20°C, purified recombinant enzyme | Moritella marina | |
0.555 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 28°C, purified recombinant enzyme | Moritella marina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by various bivalent cations | Moritella marina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Moritella marina |
60788 | - |
1 * 60788, Chi60, sequence calculation | Moritella marina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Moritella marina | chitinases hydrolyse the beta-1,4-linkages in chitin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moritella marina | B1VBB0 | i.e. Vibrio marinus, gene chi60 | - |
Purification (Comment) | Organism |
---|---|
recombinant Chi60 25fold from Escherichia coli | Moritella marina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
purified recombinant enzyme, substrate powdered chitosan | Moritella marina |
0.16 | - |
purified recombinant enzyme, substrate colloidal chitin | Moritella marina |
0.91 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | Moritella marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-1,4-N,N'-diacetyl-chitobiose + H2O | - |
Moritella marina | 4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose | - |
? | |
4-nitrophenyl beta-1,4-N,N'-diacetylchitobiose + H2O | - |
Moritella marina | ? | - |
? | |
chitin + H2O | chitinases hydrolyse the beta-1,4-linkages in chitin | Moritella marina | ? | - |
? | |
colloidal chitin + H2O | - |
Moritella marina | ? | - |
? | |
additional information | the enzyme is not active with synthetic 4-nitrophenyl-linked glycosidic substrates, overview | Moritella marina | ? | - |
? | |
powdered chitosan + H2O | - |
Moritella marina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60788, Chi60, sequence calculation | Moritella marina |
More | Chi60 domain structure, overview | Moritella marina |
Synonyms | Comment | Organism |
---|---|---|
MmChi60 | - |
Moritella marina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Moritella marina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | - |
Moritella marina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme stability at 18°C and 28°C is similar to the stability at 4°C | Moritella marina |
- |
30 | purified recombinant enzyme, 72 h, completely stable | Moritella marina |
40 | - |
purified recombinant enzyme, half-life is 72 h | Moritella marina |
50 | - |
purified recombinant enzyme, half-life is 5 h | Moritella marina |
60 | - |
purified recombinant enzyme, half-life is 10 min | Moritella marina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 0°C, purified recombinant enzyme | Moritella marina | |
4.33 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 5°C, purified recombinant enzyme | Moritella marina | |
5.46 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 10°C, purified recombinant enzyme | Moritella marina | |
7.33 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 15°C, purified recombinant enzyme | Moritella marina | |
11.88 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 20°C, purified recombinant enzyme | Moritella marina | |
19.54 | - |
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose | pH 5.0, 28°C, purified recombinant enzyme | Moritella marina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Moritella marina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | - |
Moritella marina |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified recombinant enzyme, 3 days, loss of 80% of activity at 4°C | Moritella marina |
4 | 10 | the purified recombinant Chi60 exhibits broad pH stability maintaining over 60% activity after 10 days at a pH range at 4°C | Moritella marina |
8 | 10 | purified recombinant enzyme, 10 days, no loss of activity at 4°C | Moritella marina |