Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Streptomyces sp. DA11 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Streptomyces sp. DA11 | |
EDTA | - |
Streptomyces sp. DA11 | |
EGTA | - |
Streptomyces sp. DA11 | |
Fe2+ | - |
Streptomyces sp. DA11 | |
SDS | - |
Streptomyces sp. DA11 | |
theophylline | - |
Streptomyces sp. DA11 | |
Urea | - |
Streptomyces sp. DA11 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
colloidal chitin | pH 8.0, 50°C | Streptomyces sp. DA11 | |
0.078 | - |
powdered chitin | pH 8.0, 50°C | Streptomyces sp. DA11 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates | Streptomyces sp. DA11 | |
Mg2+ | activates | Streptomyces sp. DA11 | |
Mn2+ | activates | Streptomyces sp. DA11 | |
additional information | optimal salinity for chitinase activity is 45 g psu | Streptomyces sp. DA11 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Streptomyces sp. DA11 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces sp. DA11 | the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. DA11 | B0LS89 | fragment; marine strain | - |
Purification (Comment) | Organism |
---|---|
native enzyme 6.15fold by ammonium sulfate fractionation, chitin affinity and anion exchange chromatography | Streptomyces sp. DA11 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.95 | - |
purified enzyme | Streptomyces sp. DA11 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + H2O | preferred substrate | Streptomyces sp. DA11 | ? | - |
? | |
additional information | the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity | Streptomyces sp. DA11 | ? | - |
? | |
powdered chitin + H2O | - |
Streptomyces sp. DA11 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Streptomyces sp. DA11 |
Synonyms | Comment | Organism |
---|---|---|
ChiC | - |
Streptomyces sp. DA11 |
chitinase C | - |
Streptomyces sp. DA11 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces sp. DA11 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces sp. DA11 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | stable, purified enzyme | Streptomyces sp. DA11 |