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Literature summary for 3.2.1.14 extracted from

  • Okazaki, K.; Kato, F.; Watanabe, N.; Yasuda, S.; Masui, Y.; Hayakawa, S.
    Purification and properties of two chitinase form Streptomyces sp J-13-3 (1995), Biosci. Biotechnol. Biochem., 59, 1586-1587.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Ag+ both isoenzymes Streptomyces sp.
antiserum anti-Chi-A and anti-Chi-B Streptomyces sp.
N-bromosuccinimide both isoenzymes Streptomyces sp.
N-Chlorosuccinimide both isoenzymes Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose Chi-B Streptomyces sp.
0.16
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose Chi-A Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 both isoenzymes, SDS-PAGE Streptomyces sp.
32500
-
 both isoenzymes, gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. J-13-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Chi-A 12.5fold, Chi-B 11.7fold Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.78
-
 chitopentaose, Chi-B Streptomyces sp.
5.14
-
 chitopentaose, Chi-A Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal and powdered chitin Streptomyces sp. N,N-diacetylchitobiose + N-acetylglucosamine mainly chitobiose ?
chitin + H2O colloidal and powdered chitin Streptomyces sp. J-13-3 N,N-diacetylchitobiose + N-acetylglucosamine mainly chitobiose ?
chitohexaose + H2O N-acetylglucosamine and chitobiose, both isoenzymes Streptomyces sp. chitobiose + chitotriose
-
 ?
chitohexaose + H2O N-acetylglucosamine and chitobiose, both isoenzymes Streptomyces sp. J-13-3 chitobiose + chitotriose
-
 ?
chitopentaose + H2O
-
 Streptomyces sp. chitobiose + chitotriose chitobiose and N-acetylglucosamine, both isoenzymes ?
chitopentaose + H2O
-
 Streptomyces sp. J-13-3 chitobiose + chitotriose chitobiose and N-acetylglucosamine, both isoenzymes ?
chitotetraose + H2O chitobiose, both isoenzymes Streptomyces sp. N-acetylglucosamine + chitobiose + chitotriose
-
 ?
chitotetraose + H2O chitobiose, both isoenzymes Streptomyces sp. J-13-3 N-acetylglucosamine + chitobiose + chitotriose
-
 ?
ethylene glycol chitin + H2O
-
 Streptomyces sp. ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
-
 Streptomyces sp. 5 N-acetyl-D-glucosamine
-
 ?
N,N',N''-triacetylchitotriose + H2O N-acetylglucosamine and chitobiose, both isoenzymes Streptomyces sp. N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O N-acetylglucosamine and chitobiose, both isoenzymes Streptomyces sp. J-13-3 N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 both isoenzymes Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to 45°C for 10 min, both isoenzymes Streptomyces sp.
65
-
 complete inactivation Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 both isoenzymes Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 Chi-B Streptomyces sp.
4 6 Chi-A Streptomyces sp.