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Literature summary for 3.2.1.14 extracted from

  • Konagaya, Y.; Tsuchiya, C.; Sugita, H.
    Purification and characterization of chitinases from Clostridium sp. E-16 isolated from the intestinal tract of the South American sea lion (Otaria flavescens) (2006), Lett. Appl. Microbiol., 43, 187-193.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, more than 90% inhibition, chitinase 1 Clostridium sp. E-16
Fe2+ 10 mM, more than 90% inhibition, chitinase 1; 1 mM, more than 90% inhibition, chitinase 2 Clostridium sp. E-16
Hg2+ 1 mM, more than 90% inhibition, chitinase 1 Clostridium sp. E-16
Zn2+ 1 mM, more than 90% inhibition, chitnase 1 Clostridium sp. E-16

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl more than 95% of the activity is retained at 0-8% NaCl for chitinase 2 Clostridium sp. E-16
NaCl more than 95% of the activity is retained at 0–10% NaCl for chitinase 1 Clostridium sp. E-16

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 1 * 77000, chitinase 1, SDS-PAGE Clostridium sp. E-16
98000
-
 1 * 98000, chitinase 2, SDS-PAGE Clostridium sp. E-16

Organism

Organism UniProt Comment Textmining
Clostridium sp. E-16
-
 isolated from the intestinal tract of Otaria flavescens
-

Purification (Commentary)

Purification (Comment) Organism
chitinase 1 Clostridium sp. E-16
chitinase 2 Clostridium sp. E-16

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant chitinase 1 Clostridium sp. E-16
-
culture supernatant chitinase 2 Clostridium sp. E-16
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.4
-
 chitinase 1 Clostridium sp. E-16
17.7
-
 chitinase 2 Clostridium sp. E-16

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product Clostridium sp. E-16 ?
-
 ?
additional information no activity with 4-methylumbelliferyl-(GlcNAc) Clostridium sp. E-16 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 77000, chitinase 1, SDS-PAGE Clostridium sp. E-16
monomer 1 * 98000, chitinase 2, SDS-PAGE Clostridium sp. E-16

Synonyms

Synonyms Comment Organism
chitinase 1
-
 Clostridium sp. E-16
chitinase 2
-
 Clostridium sp. E-16

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 chitinase 2 Clostridium sp. E-16
45
-
 chitinase 1 Clostridium sp. E-16

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 more than 50% of maximal activity at 20°C and at 40°C, chitinase 2 Clostridium sp. E-16
20 50 more than 50% of maximal activity at 20°C and at 50°C, chitinase 1 Clostridium sp. E-16

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 7.0, 90 min, stable Clostridium sp. E-16
45
-
 pH 7.0, 90 min, 80% loss of activity Clostridium sp. E-16
50
-
 pH 7.0, 90 min, stable, chitinase 1 Clostridium sp. E-16
55
-
 pH 7.0, 90 min, 90% loss of activity, chitinase 1 Clostridium sp. E-16

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 chitinase 1 Clostridium sp. E-16
6.5
-
 chitinase 2 Clostridium sp. E-16

pH Range

pH Minimum pH Maximum Comment Organism
4 8 about 40% of maximal activity at pH 4.0 and at pH 8.0, chitinase 1 Clostridium sp. E-16
4 8 about 40% of maximal activity at pH 4.0 and at pH 8.0, chitinase 2 Clostridium sp. E-16

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 30°C, 10 min, stable up to, chitinase 1 Clostridium sp. E-16

pI Value

Organism Comment pI Value Maximum pI Value
Clostridium sp. E-16 chitinase 1
-
 4.2
Clostridium sp. E-16 chitinase 2
-
 4.3