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Literature summary for 3.2.1.14 extracted from

  • Tsujibo, H.; Yoshida, Y.; Miyamoto, K.; Imada, C.; Okami, Y.; Inamori, Y.
    Purification, properties, and partial amino acid sequence of chitinase from a marine Alteromonas sp. strain O-7 (1992), Can. J. Microbiol., 38, 891-897.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzyl bromide
-
 Alteromonas sp.
Fe2+
-
 Alteromonas sp.
Fe3+
-
 Alteromonas sp.
N-bromosuccinimide
-
 Alteromonas sp.
Zn2+
-
 Alteromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Alteromonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activate enzyme Alteromonas sp.
K+ activates enzyme Alteromonas sp.
Mg2+ activates enzyme significantly Alteromonas sp.
Mn2+ slightly stimulates activity Alteromonas sp.
Na+ activates enzyme Alteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Alteromonas sp.

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
-
-
Alteromonas sp. O-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
4.5fold Alteromonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Alteromonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
-
 Alteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O
-
 Alteromonas sp. chitobiose + chitotriose chitobiose only ?
chitohexaose + H2O
-
 Alteromonas sp. O-7 chitobiose + chitotriose chitobiose only ?
chitopentaose + H2O
-
 Alteromonas sp. chitobiose + chitotriose chitobiose only ?
chitopentaose + H2O
-
 Alteromonas sp. O-7 chitobiose + chitotriose chitobiose only ?
chitotetraose + H2O
-
 Alteromonas sp. N-acetylglucosamine + chitobiose + chitotriose chitobiose only ?
chitotetraose + H2O
-
 Alteromonas sp. O-7 N-acetylglucosamine + chitobiose + chitotriose chitobiose only ?
N,N',N''-triacetylchitotriose + H2O
-
 Alteromonas sp. N-acetylglucosamine + N,N'-diacetylchitobiose chitobiose only ?
N,N',N''-triacetylchitotriose + H2O
-
 Alteromonas sp. O-7 N-acetylglucosamine + N,N'-diacetylchitobiose chitobiose only ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Alteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Alteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 inactivated at 60°C Alteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Alteromonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Alteromonas sp.