Cloned (Comment) | Organism |
---|---|
expression of wild-type and truncation mutant enzymes in Escherichia coli BL21(DE3) | Aeromonas caviae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of truncated mutant AcD1Chi-AK606 | Aeromonas caviae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.62 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
5.35 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
53.33 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
57.83 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aeromonas caviae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
97000 | - |
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas caviae | - |
- |
- |
Aeromonas caviae D1 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | the organism produces an extracellular chitinase when grown on the chitin as a sole carbon source | Aeromonas caviae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? | |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? | |
alpha-chitin + H2O | insoluble substrate | Aeromonas caviae | ? | - |
? | |
alpha-chitin + H2O | insoluble substrate | Aeromonas caviae D1 | ? | - |
? | |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
Synonyms | Comment | Organism |
---|---|---|
AcD1ChiA | - |
Aeromonas caviae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aeromonas caviae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
0.0012 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
0.0047 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
0.006 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aeromonas caviae |