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Literature summary for 3.2.1.14 extracted from

  • Jopcik, M.; Moravcikova, J.; Matusikova, I.; Bauer, M.; Rajninec, M.; Libantova, J.
    Structural and functional characterisation of a class I endochitinase of the carnivorous sundew (Drosera rotundifolia L.) (2017), Planta, 245, 313-327 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells Drosera rotundifolia

Localization

Localization Comment Organism GeneOntology No. Textmining
apoplast
-
 Drosera rotundifolia 48046
-

Organism

Organism UniProt Comment Textmining
Drosera rotundifolia A0A140GE99 carnivorous sundew, cultivar Vikilan Jaervi
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Drosera rotundifolia

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression Drosera rotundifolia
-
root
-
 Drosera rotundifolia
-
stem
-
 Drosera rotundifolia
-
trapping leaf highest expression in tentacles Drosera rotundifolia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside, and glycol chitin Drosera rotundifolia ?
-
 ?
N-fluorescein-labeled chitin + H2O
-
 Drosera rotundifolia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Drosera rotundifolia
? x * 31900, calculated from amino acid sequence Drosera rotundifolia
? x * 50000, recombinant enzyme fused to thioredoxin, SDS-PAGE Drosera rotundifolia

Synonyms

Synonyms Comment Organism
Chit
-
 Drosera rotundifolia
endochitinase
-
 Drosera rotundifolia

pI Value

Organism Comment pI Value Maximum pI Value
Drosera rotundifolia calculated from amino acid sequence
-
 7.5

Expression

Organism Comment Expression
Drosera rotundifolia enzyme expression is up-regulated by chitin by 7fold followed by sand (2.5fold) and gelatine (2fold), compared with expression in the non-treated (control) leaves up