Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Cho, E.K.; Choi, I.S.; Choi, Y.J.
    Overexpression and characterization of thermostable chitinase from Bacillus atrophaeus SC081 in Escherichia coli (2011), BMB Rep., 44, 193-198.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
SCChi-1, DNA and amiino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged SCChi-1 in Escherichia coli strain MC1061 Bacillus sp. SC081

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62500
-
 x * 65878, mass spectrometry, x * 62500, recombinant His6-tagged SCChi-1, SDS-PAGE Bacillus sp. SC081
65878
-
 x * 65878, mass spectrometry, x * 62500, recombinant His6-tagged SCChi-1, SDS-PAGE Bacillus sp. SC081

Organism

Organism UniProt Comment Textmining
Bacillus sp. SC081 D8V3F2 isolated from Korean traditional soy sauce
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged SCChi-1 irom Escherichia coli strain MC1061 by nickel affinity chromatography Bacillus sp. SC081

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant His6-tagged SCChi-1 shows the highest lytic activity on colloidal chitin and acidic chitosan Bacillus sp. SC081 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65878, mass spectrometry, x * 62500, recombinant His6-tagged SCChi-1, SDS-PAGE Bacillus sp. SC081

Synonyms

Synonyms Comment Organism
SCChi-1
-
 Bacillus sp. SC081

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant His6-tagged SCChi-1 Bacillus sp. SC081

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 purified recombinant His-tagged enzyme, 1 h, 80% remaining activity within this range Bacillus sp. SC081

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant His6-tagged SCChi-1 Bacillus sp. SC081

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 purified recombinant His-tagged enzyme, 1 h, 60% remaining activity within this range Bacillus sp. SC081