Literature summary for 3.2.1.14 extracted from

Songsiriritthigul, C.; Pantoom, S.; Aguda, A.H.; Robinson, R.C.; Suginta, W.
Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism (2008), J. Struct. Biol., 162, 491-499.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli	Vibrio harveyi

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged wild-type and mutant E315M mutant enzymes, free or bound to (GlcNAC)5 or (GlcNAc)6, sitting drop vapour diffusion method, 20 nl of protein solution containing 20 mg/ml wild-type protein in 10 mM Tris-HCl, pH 8.0, are mixed with 20 nl of precipitant solution, macroseeding, or 5 mg/ml protein in 1.2 M ammonium sulfate and 0.1 M Tris-HC, pH 8.0, at 14°C for 2 days, mutant E315M crystals from 20% w/v PEG 4000, 0.1 M ammonium sulfate and 0.1 M Tris-HCl, pH 7.5, 1 week at 14°C, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution	Vibrio harveyi

Crystallization (Comment)

Organism

purified recombinant His6-tagged wild-type and mutant E315M mutant enzymes, free or bound to (GlcNAC)5 or (GlcNAc)6, sitting drop vapour diffusion method, 20 nl of protein solution containing 20 mg/ml wild-type protein in 10 mM Tris-HCl, pH 8.0, are mixed with 20 nl of precipitant solution, macroseeding, or 5 mg/ml protein in 1.2 M ammonium sulfate and 0.1 M Tris-HC, pH 8.0, at 14°C for 2 days, mutant E315M crystals from 20% w/v PEG 4000, 0.1 M ammonium sulfate and 0.1 M Tris-HCl, pH 7.5, 1 week at 14°C, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution

Vibrio harveyi

Protein Variants

Protein Variants	Comment	Organism
E315M	a catalytically inactive mutant, the binding cleft of the ligand-free mutant is partially blocked by the C-terminal His6-tag, structures of E315M-chitooligosaccharide complexes, overview	Vibrio harveyi

Organism

Organism	UniProt	Comment	Textmining
Vibrio harveyi	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
chitopentaose + H2O = chitobiose + chitotriose	chitooligosaccharide-binding modes and catalytic mechanism, overview	Vibrio harveyi	a

Subunits

Subunits	Comment	Organism
More	three distinct domains: 1. the amino-terminal chitin-binding domain, 2. the main catalytic (alpha/beta)8 TIM-barrel domain, and 3. the small (alpha + beta) insertion domain. The catalytic cleft of chitinase A has a long, deep groove, which contains six chitooligosaccharide ring-binding subsites, overview	Vibrio harveyi

Synonyms

Synonyms	Comment	Organism
chitinase A	-	Vibrio harveyi
More	chitinase A belongs to the glycosyl hydrolase family 18	Vibrio harveyi