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Literature summary for 3.2.1.14 extracted from
- A gut-specific chitinase from the mulberry longicorn beetle, Apriona germari (Coleoptera: Cerambycidae): cDNA cloning, gene structure, expression and enzymatic activity (2007), Eur. J. Entomol., 104, 173-180.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| AgChi cDNA is expressed as a 46000 Da polypeptide in baculovirus-infected insect Sf9 cells and the recombinant AgChi shows activity in a chitinase enzyme assay | Apriona germari |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Apriona germari |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apriona germari | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | three N-glycosylation sites. AgChi is N-glycosylated, but the carbohydrate moieties are not essential for chitinolytic activity | Apriona germari |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | AgChi is expressed in three gut regions, indicating that the gut is the prime site for AgChi synthesis in Apriona germari larvae | Apriona germari | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Apriona germari |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgChi | - |
Apriona germari |
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