Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | 110% activity at 2 mM | Melghiribacillus thermohalophilus | |
chloroform | about 160% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
Cyclohexane | about 148% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
dimethylformamide | about % activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
DMF | about % activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
DMSO | about 160% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
Isopropanol | about 150% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
n-decane | about 110% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
n-hexane | about 130% activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
NaCl | maximum activity at 4 M | Melghiribacillus thermohalophilus |
General Stability | Organism |
---|---|
4 M NaCl. Beyond 4M NaCl, chitinase activity decreases progressively and retains 30% of its relative activity at 10 M NaCl | Melghiribacillus thermohalophilus |
more than 30% of residual activities are obtained at the range of 0.5-10 M NaCl with optimal activity reached at | Melghiribacillus thermohalophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 20% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
5,5'-dithio-bis(2-nitrobenzoic acid) | 2% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
acetonitrile | complete inhibition at 25%(v/v) | Melghiribacillus thermohalophilus | |
Ag+ | 10% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
Al3+ | 73% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
Ba2+ | 96% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
Cd2+ | complete inhibition at 2 mM | Melghiribacillus thermohalophilus | |
Cu2+ | 51% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
dithiothreitol | 8% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
ethyl acetate | complete inhibition at 25%(v/v) | Melghiribacillus thermohalophilus | |
Fe2+ | 32% residual activity at 10 mM | Melghiribacillus thermohalophilus | |
Hg2+ | complete inhibition at 2 mM | Melghiribacillus thermohalophilus | |
isooctane | about 90% residual activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
additional information | not inhibited by 2,4,6-trinitrobenzenesulfonic acid, phenylmethylsulfonyl fluoride, diethyldicarbonate, and N-bromosuccinimide | Melghiribacillus thermohalophilus | |
N-Acetylimidazole | 94% residual activity at 2 mM | Melghiribacillus thermohalophilus | |
N-ethylmaleimide | complete inhibition at 2 mM | Melghiribacillus thermohalophilus | |
n-heptane | about 80% residual activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
n-hexadecane | about 70% residual activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
Ni2+ | complete inhibition at 2 mM | Melghiribacillus thermohalophilus | |
p-chloromercuribenzoic acid | complete inhibition at 2 mM | Melghiribacillus thermohalophilus | |
Toluene | about 80% residual activity at 25%(v/v) | Melghiribacillus thermohalophilus | |
Zn2+ | 56% residual activity at 10 mM | Melghiribacillus thermohalophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 280% activity at 2 mM | Melghiribacillus thermohalophilus | |
Mg2+ | 175% activity at 2 mM | Melghiribacillus thermohalophilus | |
Mn2+ | 127% activity at 2 mM | Melghiribacillus thermohalophilus | |
additional information | not influenced by Co2+ | Melghiribacillus thermohalophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Melghiribacillus thermohalophilus | - |
- |
- |
Melghiribacillus thermohalophilus Nari2AT | - |
- |
- |
Purification (Comment) | Organism |
---|---|
UNO S-1 column chromatography and Zorbax PSM 300 HPSEC column chromatography | Melghiribacillus thermohalophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D- N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O | 535% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | 4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide | - |
? | |
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O | 198% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | 4-nitrophenyl beta-D-N,N'-diacetylchitobioside | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | 150% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
beta-D-N,N',N''-triacetylchitotriose + H2O | 86% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
chitin azure + H2O | 97% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
chitin azure + H2O | 97% activity compared to colloidal chitin | Melghiribacillus thermohalophilus Nari2AT | ? | - |
? | |
chitooligosaccharide + H2O | 77% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
colloidal chitin + H2O | 100% activity | Melghiribacillus thermohalophilus | N-acetyl-beta-D-glucosamine + ? | - |
? | |
glycol chitin + H2O | 92% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
glycol chitin + H2O | 92% activity compared to colloidal chitin | Melghiribacillus thermohalophilus Nari2AT | ? | - |
? | |
glycol chitosan + H2O | 88% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
glycol chitosan + H2O | 88% activity compared to colloidal chitin | Melghiribacillus thermohalophilus Nari2AT | ? | - |
? | |
additional information | the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin | Melghiribacillus thermohalophilus | ? | - |
? | |
additional information | the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin | Melghiribacillus thermohalophilus Nari2AT | ? | - |
? | |
xylan + H2O | 40% activity compared to colloidal chitin | Melghiribacillus thermohalophilus | ? | - |
? | |
xylan + H2O | 40% activity compared to colloidal chitin | Melghiribacillus thermohalophilus Nari2AT | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Melghiribacillus thermohalophilus |
monomer | 1 * 45201, calculated from amino acid sequence | Melghiribacillus thermohalophilus |
Synonyms | Comment | Organism |
---|---|---|
ChiA-Mt45 | - |
Melghiribacillus thermohalophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Melghiribacillus thermohalophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Melghiribacillus thermohalophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | the half-life times of the enzyme at pH 2.0, 3.0, 4.0, 5.0, and 6.0 are 18, 14, 10, 6, and 2 h, respectively | Melghiribacillus thermohalophilus |