Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20% inhibition at 5 mM | Bacillus cereus | |
Cu2+ | 27% inhibition at 5 mM | Bacillus cereus | |
EDTA | complete inhibition at 5 mM | Bacillus cereus | |
Fe2+ | complete inhibition | Bacillus cereus | |
Mg2+ | 24% inhibition at 5 mM | Bacillus cereus | |
Mn2+ | complete inhibition | Bacillus cereus | |
additional information | no inhibition by Zn2+ at 5 mM | Bacillus cereus | |
PMSF | - |
Bacillus cereus | |
SDS | 4% inhibition at 1 mM | Bacillus cereus | |
Triton X-100 | 29% inhibition at 2% | Bacillus cereus | |
Tween 20 | 40% inhibition at 2% | Bacillus cereus | |
Tween 40 | 40% inhibition at 2% | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
isolated from the soil in Taiwan | - |
Bacillus cereus TKU006 | - |
isolated from the soil in Taiwan | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation and anion exchange chromatography | Bacillus cereus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimzation of culture comditions for enzyme production, overview | Bacillus cereus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
purified enzyme | Bacillus cereus |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 60 min, completely stable | Bacillus cereus |
60 | - |
60 min, 50% remaining activity | Bacillus cereus |
80 | - |
60 min, inactivation | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | stable | Bacillus cereus |