Cloned (Comment) | Organism |
---|---|
expression in Nicotiana tabacum | Autographa californica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Autographa californica | |
Mg2+ | inhibitory above 10 mM | Autographa californica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose | 20°C, pH 6.0 | Autographa californica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5-30 mM, 10% increase in activity | Autographa californica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Autographa californica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from transgenic tobacco leaf | Autographa californica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.7 | - |
20°C, pH 6.0 | Autographa californica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O | - |
Autographa californica | 4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Autographa californica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | - |
Autographa californica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Autographa californica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Autographa californica |
10 | - |
30% residual activity | Autographa californica |
General Information | Comment | Organism |
---|---|---|
physiological function | purified ChiA enhances the permeability of the peritrophic membrane of larvae of Lepidoptera Bombyx mori and Heliothis virescens and inhibits spore germination and growth of the phytopatogenic fungus Alternaria alternata | Autographa californica |