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Literature summary for 3.2.1.14 extracted from
- An endochitinase from wheat germ. Activity on nascent and preformed chitin (1979), J. Biol. Chem., 254, 4901-4907.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diacetylchitobiose | competitive inhibitor | Triticum aestivum |  |
| N-acetylglucosamine | poor competitor | Triticum aestivum | |
| pentaacetylchitopentaose | competitive inhibitor | Triticum aestivum | |
| tetraacetylchitotetraose | competitive inhibitor | Triticum aestivum | |
| triacetylchitotriose | competitive inhibitor | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
chitin | chitin concentration calculated as N-acetylglucosamine | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Triticum aestivum |
| 30000 | - |
SDS-PAGE | Triticum aestivum |
| 33000 | - |
sedimentation equilibrium | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 300fold | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.6 | - |
calculated as N-acetylglucosamine | Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.02% azide, several weeks, no loss of activity | Triticum aestivum |
| 2°C, 0.02% azide, several weeks, no loss of activity | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | [acetyl-3H]chitin | Triticum aestivum | N,N-diacetylchitobiose + N-acetylglucosamine | chitobiose, biotriose and biotetraose from nascent chitin, higher oligosaccharides from preformed chitin | ? | |
| chitohexaose + H2O | - |
Triticum aestivum | chitobiose + chitotriose | - |
? | |
| chitopentaose + H2O | - |
Triticum aestivum | chitobiose + chitotriose | - |
? | |
| chitosan + H2O | - |
Triticum aestivum | ? | - |
? | |
| chitotetraose + H2O | tritiated | Triticum aestivum | N-acetylglucosamine + chitobiose + chitotriose | only traces of chitotriose and N-acetylglucosamine | ? | |
| glycol chitin + H2O | tritiated and unlabeled | Triticum aestivum | N-acetylglucosamine + ? | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | 3H labelled, only slightly attacked | Triticum aestivum | N-acetylglucosamine + N,N'-diacetylchitobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Triticum aestivum |
| 56 | - |
- |
Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
75% activity | Triticum aestivum |
| 9 | - |
53% activity | Triticum aestivum |
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Release 2023.1 (January 2023)
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