Literature summary for 3.2.1.14 extracted from

Mayer, R.T.; McCollum, T.G.; Niedz R.P.; Hearn, C.J.; McDonald, R.E.; Berdis, E.; Doostdar, H.
Characterization of seven basic endochitinases isolated from cell cultures of Citrus sinensis (L.) (1996), Planta, 200, 289-295.

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Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis
24200	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis
24700	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis
25000	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis
25500	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis
28000	-	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis

Organism

Organism	UniProt	Comment	Textmining
Citrus sinensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
isoenzyme 7, 0.1fold, isoenzyme 2, 3.8fold, isoenzyme 6, 0.1fold, isoenzyme 1, 6.2fold, isoenzyme 5, 0.1fold, isoenzyme 4, 1.6fold, isoenzyme 3, 0.004fold	Citrus sinensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
callus	nonembryonic, cell cultures	Citrus sinensis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0025	-	isoenzyme 3	Citrus sinensis
0.0097	-	isoenzyme 6	Citrus sinensis
0.0127	-	isoenzyme 7	Citrus sinensis
0.0193	-	isoenzyme 4	Citrus sinensis
0.0391	-	isoenzyme 2	Citrus sinensis
0.0421	-	isoenzyme 5	Citrus sinensis
0.2291	-	isoenzyme 1	Citrus sinensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethylated-chitin-remazol brilliant violet + H2O	-	Citrus sinensis	?	-	?
chitin + H2O	[acetyl-3H]chitin	Citrus sinensis	N,N-diacetylchitobiose + N-acetylglucosamine	-	?
chitohexaose + H2O	-	Citrus sinensis	chitobiose + chitotriose	chitobiose and chitotetraose	?
chitopentaose + H2O	-	Citrus sinensis	chitobiose + chitotriose	chitobiose, chitotetraose and trace amounts of chitotriose	?
chitosan + H2O	different percentages of acetylation	Citrus sinensis	chitosan oligosaccharides	-	?
chitotetraose + H2O	-	Citrus sinensis	N-acetylglucosamine + chitobiose + chitotriose	chitobiose and chitotetraose	?

Subunits

Subunits	Comment	Organism
?	x * 28000, isoenzyme 1, SDS-PAGE, x * 25500, isoenzyme 2, SDS-PAGE, x * 23000, isoenzyme 3, SDS-PAGE, x * 24200, isoenzyme 4, SDS-PAGE, x * 24700, isoenzyme 5, SDS-PAGE, x * 25000, isoenzyme 6, SDS-PAGE, x * 25000, isoenzyme 7, SDS-PAGE	Citrus sinensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	-	Citrus sinensis
4	4.5	isoenzyme 7	Citrus sinensis
5	-	isoenzymes 1,2 and 5	Citrus sinensis
5	5.5	isoenzymes 4 and 6	Citrus sinensis
5	5.2	chitosanase activity, isoenzymes 1-7	Citrus sinensis
5.5	-	isoenzyme 3	Citrus sinensis

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Release 2023.1 (January 2023)