Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | deletorious effect at 0.01 M | Streptomyces sp. | |
Co2+ | inhibiting above 1 mM | Streptomyces sp. | |
Mg2+ | - |
Streptomyces sp. | |
NaCl | - |
Streptomyces sp. | |
Zn2+ | inhibiting above 1 mM | Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | necessary in small amounts in order to retain enyzmatic activity during purification | Streptomyces sp. | |
Co2+ | activating below 1 mM | Streptomyces sp. | |
Zn2+ | activating below 1 mM | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
equilibrium sedimentation | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. 2B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.289 | - |
- |
Streptomyces sp. |
Storage Stability | Organism |
---|---|
2°C, lycophilized, after purifiaction step 5, stable | Streptomyces sp. |
frozen, prolonged periods, after purification step 4, stable | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Streptomyces sp. | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylhexosamine | ? | |
chitin + H2O | - |
Streptomyces sp. 2B | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylhexosamine | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
loss of all activity after 3 h | Streptomyces sp. |
70 | - |
loss of all activity after 5 min, 80% of activity remains when dried | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
Na-phosphate buffer | Streptomyces sp. |
4.8 | - |
Na-acetate buffer, sodium phosphate-acetate buffer | Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
11 | - |
inactivated above pH 11 | Streptomyces sp. |