Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 110% activity at 5 mM | Stenotrophomonas maltophilia | |
EDTA | 110% activity at 5 mM | Stenotrophomonas maltophilia | |
L-cysteine | 105% activity at 1 mM | Stenotrophomonas maltophilia | |
Triton X-100 | 104% activity at 1 mM | Stenotrophomonas maltophilia | |
Tween 20 | 105% activity at 5 mM | Stenotrophomonas maltophilia | |
Tween 80 | 110% activity at 5 mM | Stenotrophomonas maltophilia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 80% residual activity at 5 mM | Stenotrophomonas maltophilia | |
Hg2+ | 10% residual activity at 5 mM | Stenotrophomonas maltophilia | |
NH4+ | 30% residual activity at 5 mM | Stenotrophomonas maltophilia | |
phenylmethylsulfonyl fluoride | 64% residual activity at 5 mM | Stenotrophomonas maltophilia | |
SDS | 25% residual activity at 5 mM | Stenotrophomonas maltophilia | |
Zn2+ | 90% residual activity at 5 mM | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside | at pH 5.6 and 45°C | Stenotrophomonas maltophilia | |
3.9 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | at pH 5.6 and 45°C | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ and Ca2+ do not affect the activity of the enzyme | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | Stenotrophomonas maltophilia | highest activity | 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | Stenotrophomonas maltophilia N4 | highest activity | 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | Stenotrophomonas maltophilia | - |
4-nitrophenol + beta-D-N,N'-diacetylchitobiose | - |
? | |
chitin flakes + H2O | Stenotrophomonas maltophilia | 10.8% activity compared to colloidal chitin | N-acetyl-beta-D-glucosamine + ? | - |
? | |
chitin powder + H2O | Stenotrophomonas maltophilia | 32.5% activity compared to colloidal chitin | N-acetyl-beta-D-glucosamine + ? | - |
? | |
chitosan + H2O | Stenotrophomonas maltophilia | 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin | ? | - |
? | |
chitosan + H2O | Stenotrophomonas maltophilia N4 | 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin | ? | - |
? | |
colloidal chitin + H2O | Stenotrophomonas maltophilia | 100% activity | N-acetyl-beta-D-glucosamine + ? | - |
? | |
colloidal chitin + H2O | Stenotrophomonas maltophilia N4 | 100% activity | N-acetyl-beta-D-glucosamine + ? | - |
? | |
glycol chitin + H2O | Stenotrophomonas maltophilia | 29.8% activity compared to colloidal chitin | N-acetyl-beta-D-glucosamine + ? | - |
? | |
laminarin + H2O | Stenotrophomonas maltophilia | the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin | ? | - |
? | |
laminarin + H2O | Stenotrophomonas maltophilia N4 | the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin | ? | - |
? | |
additional information | Stenotrophomonas maltophilia | the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide | ? | - |
? | |
additional information | Stenotrophomonas maltophilia N4 | the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Stenotrophomonas maltophilia N4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and Superdex 200 gel filtration | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Stenotrophomonas maltophilia | 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Stenotrophomonas maltophilia N4 | 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Stenotrophomonas maltophilia | 4-nitrophenol + beta-D-N,N'-diacetylchitobiose | - |
? | |
chitin flakes + H2O | 10.8% activity compared to colloidal chitin | Stenotrophomonas maltophilia | N-acetyl-beta-D-glucosamine + ? | - |
? | |
chitin powder + H2O | 32.5% activity compared to colloidal chitin | Stenotrophomonas maltophilia | N-acetyl-beta-D-glucosamine + ? | - |
? | |
chitosan + H2O | 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin | Stenotrophomonas maltophilia | ? | - |
? | |
chitosan + H2O | 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin | Stenotrophomonas maltophilia N4 | ? | - |
? | |
colloidal chitin + H2O | 100% activity | Stenotrophomonas maltophilia | N-acetyl-beta-D-glucosamine + ? | - |
? | |
colloidal chitin + H2O | 100% activity | Stenotrophomonas maltophilia N4 | N-acetyl-beta-D-glucosamine + ? | - |
? | |
glycol chitin + H2O | 29.8% activity compared to colloidal chitin | Stenotrophomonas maltophilia | N-acetyl-beta-D-glucosamine + ? | - |
? | |
laminarin + H2O | the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin | Stenotrophomonas maltophilia | ? | - |
? | |
laminarin + H2O | the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin | Stenotrophomonas maltophilia N4 | ? | - |
? | |
additional information | the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Stenotrophomonas maltophilia | ? | - |
? | |
additional information | the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Stenotrophomonas maltophilia N4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
endochitinase | - |
Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Stenotrophomonas maltophilia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | more than 60% activity between 30 and 55°C | Stenotrophomonas maltophilia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme demonstrates thermal stability with 80% activity remaining at 45°C within 2 h | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Stenotrophomonas maltophilia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.4 | 7.8 | more than 60% activity between pH 4.4 and 7.8 | Stenotrophomonas maltophilia |