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Literature summary for 3.2.1.14 extracted from
- Overexpression, purification and characterization of the Trichoderma atroviride endochitinase, Ech42, in Pichia pastoris (2007), Protein Expr. Purif., 55, 183-188.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Pichia pastoris using a constitutive expression system. The optimal enzyme kinetic parameters for the recombinant protein were identical to those reported for the enzyme isolated from Trichoderma atroviride | Trichoderma atroviride |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
colloidal chitin | - |
Trichoderma atroviride |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Trichoderma atroviride |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma atroviride | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trichoderma atroviride |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichoderma atroviride |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Trichoderma atroviride | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + H2O | - |
Trichoderma atroviride | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Trichoderma atroviride |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ech42 | - |
Trichoderma atroviride |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trichoderma atroviride |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | 10°C: about 50% of maximal activity, 60°C: about 59% of maximal activity | Trichoderma atroviride |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Trichoderma atroviride |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | pH 3.0: about 35% of maximal activity, pH 7.0: about 60% of maximal activity | Trichoderma atroviride |
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