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(-)-carvone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
(1S,3S)-3-methylcyclohexanol + NAD+
(rac)-3-methylcyclohexanone + NADH + H+
125% activity compared to cyclohexanol
163% activity compared to cyclohexanone
-
r
(2E)-2-methylpent-2-enal + NADPH + H+
(2E)-2-methylpent-2-en-1-ol + NADP+
41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h
-
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+
(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h
-
-
?
(2E)-4-hydroxynon-2-enal + NADH + H+
(2E)-non-2-ene-1,4-diol + NAD+
-
substrate of isozyme ADH4
-
-
r
(2E)-but-2-en-1-ol + NADP+
(2E)-but-2-enal + NADPH + H+
-
-
-
r
(2E)-but-2-enal + NADPH + H+
(2E)-but-2-en-1-ol + NADP+
yield 96% after 12 h
-
-
r
(2E)-dec-2-enal + NADPH + H+
(2E)-dec-2-en-1-ol + NADP+
16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h
-
-
?
(2E)-hex-2-enal + NADPH + H+
(2E)-hex-2-en-1-ol + NADP+
41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h
-
-
?
(2E)-oct-2-enal + NADPH + H+
(2E)-oct-2-en-1-ol + NADP+
30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h
plus 27% oct-2-enyl ester
-
?
(6S)-5,6-dihydro-6-methyl-4H-thieno[2,3b]thiopyran-4-one-7,7-dioxide + NADH + H+
(4S,6S)-5,6-dihydro-4-hydroxy-6-methyl-4H-thieno[2,3b]thiopyran-7,7-dioxide + NAD+
-
-
-
-
?
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
(R)-1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
r
(R)-1-indanol + NAD+
?
62% of activity compared to (S)-1-indanol
-
-
?
(R)-1-phenyl-1-butanol + NAD+
(R)-1-phenylbutan-1-one + NADH + H+
(R)-1-phenyl-1-propanol + NAD+
(R)-1-phenyl-1-propanone + NADH + H+
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
(R)-2-butanol + NAD+
2-butanone + NADH + H+
(R)-2-butanol + NAD+
butanone + NADH + H+
-
-
-
-
r
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
(R)-2-octanol + NAD+
2-octanone + NADH + H+
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
(R)-3-methylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
(R)-4-phenyl-2-butanol + NAD+
(R)-4-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(R)-4-phenyl-3-butanol + NAD+
(R)-4-phenylbutan-3-one + NADH + H+
-
-
-
-
r
(R)-alpha-tetralol + NAD+
?
58% of activity compared to (S)-1-indanol
-
-
?
(R)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
(S)-(+)-1-indanol + NAD+
indanone + NADH + H+
-
-
-
-
?
(S)-1-indanol + NAD+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
?
-
-
-
?
(S)-1-phenyl-1-butanol + NAD+
(S)-1-phenylbutan-1-one + NADH + H+
-
no substrate of mutant B1
-
-
r
(S)-1-phenyl-1-propanol + NAD+
(S)-1-phenyl-1-propanone + NADH + H+
-
-
-
-
r
(S)-1-phenyl-2-propanol + NAD+
phenylacetone + NADH + H+
-
9% activity compared to cyclohexanone
-
r
(S)-1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
-
-
-
-
r
(S)-1-phenylethanol + NAD+
acetophenone + NADH + H+
(S)-2-butanol + NAD+
2-butanone + NADH
-
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
(S)-2-butanol + NAD+
butanone + NADH + H+
(S)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
331% of the activity with 2-propanol
-
-
r
(S)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
38% of the activity with 2-propanol
-
-
r
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
(S)-2-octanol + NAD+
2-octanone + NADH + H+
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
(S)-2-pentanol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
(S)-2-phenylpropanol + NAD+
(S)-2-phenylpropanal + NADH + H+
-
156% of the activity with 2-phenylethanol
-
-
?
(S)-4-phenyl-2-butanol + NAD+
(S)-4-phenylbutan-2-one + NADH + H+
-
-
-
-
r
(S)-4-phenyl-3-butanol + NAD+
(S)-4-phenylbutan-3-one + NADH + H+
-
-
-
-
r
(S)-4-phenylbutan-2-ol + NAD+
benzylacetone + NADH + H+
-
3% activity compared to cyclohexanone
-
r
(S)-alpha-tetralol + NAD+
?
12% of activity compared to (S)-1-indanol
-
-
?
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
(S)-heptan-2-ol + NAD+
2-heptanone + NADH + H+
-
-
-
r
(S)-pentan-2-ol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(S)-perillylalcohol + NAD+
(S)-perillaldehyde + NADH + H+
52% activity compared to cyclohexanol
-
-
?
(Z)-hex-2-en-1-ol + NAD+
(Z)-hex-2-en-1-one + NADH
-
9.7% of the activity with ethanol
-
-
?
1,1-dichloroacetone + NADH + H+
1,1-dichloropropan-2-ol + NAD+
-
1078% of the activity with phenyl trifluoromethyl ketone
-
-
?
1,2-butanediol + NAD+
? + NADH + H+
1,2-hexanediol + NAD+
?
-
20% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-pentanediol + NAD+
?
-
12% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
-
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH + H+
-
-
-
-
?
1,3-butanediol + NAD+
?
-
39% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,3-propanediol + 2 NAD+ + H2O
propanedial + 2 NADH + 2 H+
-
-
-
r
1,3-propanediol + NAD+
? + NADH
-
7% of the activity with ethanol
-
-
?
1,3-propanediol + NAD+
? + NADH + H+
1,4-butanediol + NAD+
? + NADH + H+
best substrate in oxidation reaction
-
-
?
1,5-pentanediol + NAD+
? + NADH + H+
18% of the activity with butan-1-ol
-
-
?
1-(3-bromophenyl)ethanol + NAD+
1-(3-bromophenyl)ethanone + NADH + H+
315% of the activity with 1-phenylethanol
-
-
?
1-(3-chlorophenyl)ethanol + NAD+
1-(3-chlorophenyl)ethanone + NADH + H+
205% of the activity with 1-phenylethanol
-
-
?
1-(4'-chlorophenyl)ethanol + NAD+
1-(4'-chlorophenyl)ethanone + NADH + H+
-
26% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-(4'-fluorophenyl)ethanol + NAD+
1-(4'-fluorophenyl)ethanone + NADH + H+
-
45% of the activity with (S)-1-phenylethanol
-
-
r
1-(4-bromophenyl)ethanol + NAD+
1-(4-bromophenyl)ethanone + NADH + H+
167% of the activity with 1-phenylethanol
-
-
?
1-(4-chlorophenyl)ethanol + NAD+
1-(4-chlorophenyl)ethanone + NADH + H+
151% of the activity with 1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
1-(4-methylphenyl)ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
189% of the activity with 1-phenylethanol
-
-
?
1-(p-tolyl)-ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
19% activity compared to cyclohexanol
26% activity compared to cyclohexanone
-
?
1-butanal + NADH + H+
1-butanol + NAD+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH
1-butanol + NAD+
butanal + NADH + H+
1-butanol + NAD+
butyraldehyde + NADH + H+
1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+
1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
1-decalone + NADH + H+
decahydronaphthalen-1-ol + NAD+
85% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
1-decanol + NAD+
decanal + NADH + H+
1-dodecanol + NAD+
dodecanal + NADH
1-heptanol + NAD+
1-heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
1-hexanal + NADH + H+
1-hexanol + NAD+
-
-
-
-
r
1-hexanol + NAD+
1-hexanal + NADH + H+
1-hexanol + NAD+
?
-
15% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
1-hydroxy-2-butanone + NADH + H+
butane-1,2-diol + NAD+
-
59% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
1-hydroxymethyl-6-methylpyrene + NAD+
1-formyl-6-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethyl-8-methylpyrene + NAD+
1-formyl-8-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethylpyrene + NAD+
1-formylpyrene + NADH + H+
-
-
-
r
1-indanol + NAD+
1-indanone + NADH + H+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
1-indanone + NADH + H+
1-indanol + NAD+
-
-
-
-
r
1-nonanol + NAD+
nonanal + NADH + H+
1-octanol + NAD+
1-octanal + NADH + H+
-
6% of activity with N-benzyl-3-pyrrolidinol
-
-
?
1-octanol + NAD+
octanal + NADH
1-octanol + NAD+
octanal + NADH + H+
1-pentanol + NAD+
1-pentanal + NADH + H+
1-pentanol + NAD+
pentanal + NADH + H+
1-phenyl-1,2-ethanediol + NAD+
1-phenyl-2-propanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
1-phenyl-1,2-propandione + NADH + H+
1-phenyl-2-hydroxy-1-propanone + NAD+
-
146% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
1-phenyl-1,2-propanedione + NADH
?
about 25% of the activity compared to isatin
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1-propanol + NAD+
1-phenyl-1-propanone + NADH + H+
-
59% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-phenyl-1-propanol + NAD+
1-phenylpropan-1-one + NADH + H+
-
59% of the activity with (S)-1-phenylethanol
-
-
r
1-phenyl-1-propanol + NAD+
?
-
31% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
1-phenyl-2-propanone + NADH + H+
(S)-1-phenyl-2-propanol + NAD+
-
-
-
-
?
1-phenyl-3-butanone + NADH + H+
1-phenylbutan-3-ol + NAD+
-
353% of the activity with phenyl trifluoromethyl ketone
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADH + H+
-
1% activity compared to cyclohexanone
-
?
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
-
-
-
?
1-propanol + NAD+
propanal + NADH
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
1-propanol + NAD+
propanaldehyde + NADH + H+
1-propanol + NAD+
propionaldehyde + NADH + H+
-
-
-
?
11-cis-retinal + NADH + H+
11-cis-retinol + NAD+
-
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
12-hydroxylauric acid methyl ester + NAD+
12-oxolauric acid methyl ester + NADH + H+
-
product is a key intermediate for biobased polyamide 12 production
-
?
12-oxolauric acid methyl ester + NADH + H+
12-hydroxylauric acid methyl ester + NAD+
-
-
-
?
13-cis-retinal + NADH + H+
13-cis-retinol + NAD+
-
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
16-hydroxyhexadecanoate + NAD+
16-oxohexadecanoic acid + NADH
-
-
-
-
?
17beta-hydroxyetiocholan-3-one + NAD+
ethiocholan-3,17-dione + NADH
-
-
-
-
?
2',3',4',5',6'-pentafluoroacetophenone + NADH + H+
1-(2,3,4,5,6-pentafluorophenyl)ethanol + NAD+
45% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2'-dichlorobenzil + NADH + H+
1,2-bis(2-chlorophenyl)-2-hydroxyethanone + NAD+
-
-
-
?
2,2,2-trichloroethanol + NAD+
trichloroacetaldehyde + NADH + H+
-
-
-
-
?
2,2,2-trifluoro-1-phenylethanone + propan-2-ol
(1R)-2,2,2-trifluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2,2,2-trifluoroacetophenone + NADH
2,2,2-trifluoro-1-phenylethanol + NAD+
about 35% of the activity compared to isatin
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
2,2,2-trifluoroacetophenone + NADPH + H+
(R)-2,2,2-trifluoro-1-phenylethanol + NADP+
180% of the activity with acetoin
-
-
r
2,2,2-trifluoroethanol + NAD+
2,2,2-trifluoroethanone + NADH + H+
-
-
-
?
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
2,2-difluoro-1-phenylethanone + propan-2-ol
(1R)-2,2-difluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 94% enantiomeric excess
-
?
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
2,3,4,5,6-pentafluorobenzyl alcohol + NAD+
2,3,4,5,6-pentafluorobenzaldehyde + NADH + H+
-
-
-
?
2,3-butanediol + H2O
?
-
-
-
?
2,3-butanediol + NAD+
3-hydroxy-2-butanone + NADH + H+
-
-
-
-
r
2,3-butanediol + NAD+
?
-
83% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
2,3-butanediol + NADP+
3-hydroxy-2-butanone + NADPH + H+
-
-
-
-
?
2,3-butanediol + NADP+
acetoin + NADPH + H+
2,3-butanedione + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2,3-pentanedione + NADH + H+
? + NAD+
-
5.5% of the activity with acetaldehyde
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
2-(4-trifluoromethylphenyl)propanal + NADH + H+
(S)-2-(4-trifluoromethyl)phenylpropanol + NAD+
18 h, 55% yield, 98% enantiomeric excess
-
-
?
2-(6-methoxynaphthalen-2-yl)propanal + NADH + H+
(S)-2-(6-methoxynaphthalen-2-yl)propan-1-ol + NAD+
18 h, 96% yield, 98% enantiomeric excess
-
-
?
2-(naphthalen-1-yl)propanal + NADH + H+
(S)-2-(naphthalen-1-yl)propanol + NAD+
18 h, 90% yield, 80% enantiomeric excess
-
-
?
2-(naphthalen-2-yl)propanal + NADH + H+
(S)-2-(naphthalen-2-yl)propanol + NAD+
18 h, 57% yield, 94% enantiomeric excess
-
-
?
2-acetylcyclohexanone + NADH + H+
2-acetylcyclohexanol + NAD+
-
-
-
-
r
2-acetylcyclopentanone + NADH + H+
2-acetylcyclopentanol + NAD+
-
-
-
-
r
2-acetylfuran + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylpyridine + NADH + H+
(R)-1-(2-pyridyl)ethanol + NAD+
-
-
-
-
r
2-acetylpyrrole + NADH + H+
1-(1H-pyrrol-2-yl)ethanol + NAD+
-
70% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-acetylpyrrole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiazole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiophene + NADH + H+
? + NAD+
-
-
-
-
r
2-aminoethanol + NAD+
?
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
2-butanone + NADH + H+
2-butanol + NAD+
2-butanone + NADH + H+
butan-2-ol + NAD+
-
2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-butanone + NADPH + H+
butan-2-ol + NADP+
12.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-butene-1-ol + NAD+
? + NADH
2-chloro-1-(2,4-dichlorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol + NAD+
AE010289.1
production of with (1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol 99% enantiomeric excess
-
-
?
2-chloro-1-(2,4-difluorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol + NAD+
AE010289.1
production of (1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol with 99% enantiomeric excess
-
-
?
2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+
(1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+
AE010289.1
production of (1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol with 98% enantiomeric excess
-
-
?
2-chloroacetophenone + NADH + H+
1-(2-chlorophenyl)ethanol + NAD+
-
low activity
-
-
r
2-chlorocyclohexanone + NADH + H+
? + NAD+
3% activity compared to cyclohexanone
-
-
?
2-decalone + NADH + H+
? + NAD+
28% activity compared to cyclohexanone
-
-
?
2-decanol + NAD+
2-decanone + NADH + H+
weak activity
-
-
r
2-decanone + NADH
(S)-2-decanol + NAD+ + H+
-
92% enantiomeric excess
-
?
2-dehydro-3-deoxy-D-gluconate + NADH + H+
4-deoxy-L-erythro-5-hexoseulose + NAD+
-
-
-
r
2-deoxy-D-ribose + NAD+
? + NADH
-
-
-
-
?
2-ethoxyethanol + NAD+
2-ethoxyacetaldehyde + NADH + H+
-
-
-
r
2-ethylhexan-1-ol + NAD+
2-ethylhexanal + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
2-fluoro-1-(4-chlorophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-chlorophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 98% enantiomeric excess
-
?
2-fluoro-1-(4-fluorophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-fluorophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 97% enantiomeric excess
-
?
2-fluoro-1-(4-iodophenyl)ethanone + propan-2-ol
(1R)-2-fluoro-1-(4-iodophenyl)ethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-phenylethanone + propan-2-ol
(1R)-2-fluoro-1-phenylethanol + acetone
in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH
99% conversion, 99% enantiomeric excess
-
?
2-heptanol + NAD+
2-heptanone + NADH + H+
2-heptanol + NAD+
heptan-2-one + NADH + H+
62% of the activity with 1-phenylethanol
-
-
?
2-heptanone + NADH
(S)-2-heptanol + NAD+ + H+
-
79% enantiomeric excess
-
?
2-heptanone + NADH + H+
(R)-2-heptanol + NAD+
-
229% of the activity with phenyl trifluoromethyl ketone
99% enantiomeric excess
-
?
2-heptanone + NADPH + H+
heptan-2-ol + NADP+
6.8% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hexanol + NAD+
2-hexanone + NADH + H+
2-hexanone + NADH
(S)-2-hexanol + NAD+ + H+
-
37% enantiomeric excess
-
?
2-hexanone + NADH + H+
2-hexanol + NAD+
-
-
-
-
r
2-hexanone + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADH + H+
hexan-2-ol + NAD+
-
50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADPH + H+
hexan-2-ol + NADP+
5.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hydroxyacetophenone + NADH + H+
(S)-1-phenyl-1,2-ethanediol + NAD+
2.4% of the activity with NADPH
-
-
?
2-hydroxyacetophenone + NADPH + H+
(S)-1-phenyl-1,2-ethanediol + NADP+
-
-
-
?
2-hydroxymethylpyrene + NAD+
2-formylpyrene + NADH + H+
-
-
-
r
2-mercaptoethanol + NAD+
?
2-methoxybenzaldehyde + NADH + H+
2-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
2-methoxybenzyl alcohol + NAD+
2-methoxybenzaldehyde + NADH + H+
-
25% of the activity with (S)-(-)-1-phenylethanol
-
-
?
2-methoxyethanol + NAD+
2-methoxyacetaldehyde + NADH + H+
-
-
-
r
2-methyl-2,4-pentanediol + NAD+
?
1% activity compared to cyclohexanol
-
-
?
2-methylbutan-1-ol + NAD+
? + NADH
-
-
-
-
?
2-methylbutyraldehyde + NADH + H+
2-methylbutanol + NAD+
-
1.4% of the activity with acetaldehyde
-
-
?
2-methylcyclohexanol + NAD+
(+)-2-methylcyclohexanone + NADH + H+
-
-
-
-
r
2-methylcyclohexanone + NADH + H+
2-methylcyclohexanol + NAD+
13% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2-methylcyclohexanone + NADH + H+
? + NAD+
46% activity compared to cyclohexanone
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
2-methylpropionaldehyde + NADH + H+
2-methylpropanal + NAD+
-
3.3% of the activity with acetaldehyde
-
-
?
2-nitrobenzaldehyde + NADH + H+
(2-nitrophenyl)methanol + NAD+
-
33% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-nitrobenzaldehyde + NADH + H+
2-nitrobenzyl alcohol + NAD+
-
low activity
-
-
r
2-nonanol + NAD+
2-nonanone + NADH + H+
weak activity
-
-
r
2-nonanone + NADH
(S)-2-nonanol + NAD+ + H+
-
95% enantiomeric excess
-
?
2-octanol + NAD+
2-octanone + NADH + H+
2-octanone + NADH
(S)-2-octanol + NAD+ + H+
-
92% enantiomeric excess
-
?
2-octanone + NADH + H+
2-octanol + NAD+
-
-
-
-
r
2-octanone + NADPH + H+
octan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
2-oxo-4-methylpentane + NADH
4-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-butyric acid + NADH + H+
2-hydroxybutyric acid + NAD+
-
15% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-oxohexane + NADH
2-hydroxyhexane + NAD+
-
-
-
-
?
2-oxopentane + NADH
2-pentanol + NAD+
-
-
-
-
?
2-oxopentanoate + NADH + H+
2-hydroxypentanoate + NAD+
-
low activity
-
-
r
2-oxopropanal + NADH + H+
? + NAD+
328% of the activity with acetoin
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
2-pentanone + NADH + H+
(S)-2-pentanol + NAD+
-
60% enantiomeric excess
-
?
2-pentanone + NADH + H+
2-pentanol + NAD+
2-pentanone + NADH + H+
pentan-2-ol + NAD+
-
-
-
r
2-pentanone + NADPH + H+
pentan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-phenylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
2-phenylethanol + NAD+
2-phenylethanone + NADH + H+
57% activity compared to cyclohexanol
-
-
r
2-phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
-
-
-
r
2-phenylpropanal + NADH + H+
(S)-2-phenylpropanol + NAD+
18 h, 74% yield, 98% enantiomeric excess
-
-
?
2-phenylpropionaldehyde + NADH + H+
2-phenylpropanol + NAD+
-
-
-
-
r
2-propanol + NAD(P)+
acetone + NAD(P)H
2-propanol + NAD+
2-propanone + NADH
2-propanol + NAD+
2-propanone + NADH + H+
2-propanol + NAD+
aceton + NADH + H+
-
-
irreversible, no measurable activity with acetone
-
ir
2-propanol + NAD+
acetone + NADH
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
propanone + NADH + H+
-
-
-
-
r
2-[3-(2-phenyl-1,3-dioxolan-2-yl)phenyl]propanal + NADH + H+
(S)-2-(3-(2-phenyl-1,3-dioxolan-2-yl)phenyl)propanol + NAD+
18 h, 95% yield, 61% enantiomeric excess
-
-
?
3',4'-dimethoxyphenylacetone + NADH + H+
1-(3,4-dimethoxyphenyl)propan-2-ol + NAD+
-
24% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-bromoacetophenone + NADH + H+
1-(3-bromophenyl)ethanol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-chloroacetophenone + NADH + H+
1-(3-chlorophenyl)ethanol + NAD+
-
70% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-methoxyacetophenone + NADH + H+
1-(3-methoxyphenyl)ethanol + NAD+
-
51% of the activity with phenyl trifluoromethyl ketone
-
-
?
3,3,5-trimethylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3,4-dihydro-retinol + NAD+
3,4-dihydro-retinal
-
-
-
-
r
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH + H+
3,4-hexanedione + NADH + H+
?
-
77% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
3,4-methylenedioxyphenyl acetone + NADH
(S)-(3,4-methylenedioxyphenyl)-2-propanol + NAD+ + H+
-
-
-
-
?
3,5-dimethylcyclohexanol + NAD+
3,5-dimethylcyclohexanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
3-acetylpyridine + NADH + H+
(R)-1-(3-pyridyl)ethanol + NAD+
-
-
-
-
r
3-acetylpyridine + NADH + H+
1-pyridin-3-ylethanol + NAD+
-
7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-aminobenzyl alcohol + NAD+
3-aminobenzaldehyde + NADH + H+
8% activity compared to cyclohexanol
-
-
r
3-bromobenzyl alcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-bromobenzylalcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
3-chloro-2-butanone + NADH + H+
3-chlorobutan-2-ol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-chlorobenzaldehyde + NADH + H+
3-chlorobenzyl alcohol + NAD+
-
-
-
-
r
3-heptanol + NAD+
3-heptanone + NADH + H+
-
93% of the activity with 2-propanol
-
-
r
3-hydroxy-2-butanone + NADH + H+
2,3-butanediol + NAD+
-
-
-
-
r
3-hydroxy-2-butanone + NADPH + H+
2,3-butanediol + NADP+
-
-
-
-
r
3-methoxy-1-phenylpropan-1-one + NADH
3-methoxy-1-phenylpropan-1-ol + NAD+
-
-
-
-
?
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzyl alcohol + NAD+
-
-
-
-
r
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methyl-1-butanol + NAD+
? + NADH
-
-
-
-
?
3-methyl-2-cyclohexenone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
3-methylbutan-2-ol + NAD+
3-methylbutan-2-one + NADH
-
R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol
-
-
?
3-methylbutan-2-one + NADH
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutan-2-one + NADH + H+
3-methyl-2-butanol + NAD+
-
-
-
-
r
3-methylbutan-2-one + NADH + H+
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
3-methylbutanal + NADPH + H+
3-methylbutanol + NADP+
low activity, reaction of EC 1.1.1.2
-
-
ir
3-methylbutanol + NAD+
3-methylbutanone + NADH + H+
133% activity compared to cyclohexanol
-
-
r
3-methylbutanol + NAD+
? + NADH
3-methylbutyraldehyde + NADH + H+
3-methylbutanol + NAD+
-
2.9% of the activity with acetaldehyde
-
-
?
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
-
-
-
-
r
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
14% of activity compared to (S)-1-indanol
-
-
?
3-methylcyclohexanol + NAD+
?
10% of the activity compared to isoborneol
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
3-methylcyclohexanol + NAD+
33% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
3-methylphenylethyl alcohol + NAD+
?
64% activity compared to cyclohexanol
-
-
?
3-nitroacetophenone + NADH + H+
1-(3-nitrophenyl)ethanol + NAD+
-
-
-
-
r
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
3-oxoheptane + NADH
3-hydroxyheptane + NAD+
-
-
-
-
?
3-pentanol + NAD+
3-pentanone + NADH + H+
3-pentanone + NADH
pentan-3-ol + NAD+
-
-
-
r
3-pentanone + NADPH + H+
pentan-3-ol + NADP+
7.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
3-penten-2-one + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3-phenyl-1-propanol + NAD+
3-phenyl-1-propanone + NADH
-
-
-
-
?
3-phenyl-2-propen-1-ol + NAD+
? + NADH
-
-
-
-
?
3-phenylpropanol + NAD+
3-phenylpropanal + NADH + H+
-
135% of the activity with 2-phenylethanol
-
-
?
3-phenylpropionaldehyde + NADH
3-phenylpropan-1-ol + NAD+
-
1218% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-phenylpropionaldehyde + NADH + H+
3-phenylpropanol + NAD+
-
6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-pyridylcarbinol + NAD+
pyridine-3-carbaldehyde + NADH
-
-
-
-
?
3beta,12alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,17beta-dihydroxy-5beta-androstane + NAD+
5beta-androstan-3,17dione + NADH
-
-
-
-
?
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta-7alpha-dihydroxy-5beta-cholanoate + NAD+
17-hydroxy-3-oxo-5beta-cholanoate + NADH + H+
-
-
-
-
r
3beta-hxdroxy-5alpha-cholanoate + NAD+
3-oxo-5alpha-cholanoate + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
3beta-hydroxy-5beta-cholanoate + NAD+
3-oxo-5beta-cholanoate + NADH
-
-
-
-
?
4'-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
77% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
60% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chlorobutyrophenone + NADH + H+
1-(4-chlorophenyl)butan-1-ol + NAD+
-
10% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-acetylpyridine + NADH + H+
(R)-1-(4-pyridyl)ethanol + NAD+
-
-
-
-
r
4-acetylpyridine + NADH + H+
1-pyridin-4-ylethanol + NAD+
-
64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
4-amino-1-butanol + NAD+
to gamma-butyrolactam + NADH + H+
95% conversion
-
-
?
4-bromoacetophenone + NADH + H+
4-bromo-1-phenylethanol + NAD+
4-bromobenzyl alcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-bromobenzylalcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzyl alcohol + NAD+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzylalcohol + NAD+
-
-
-
-
?
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
4-chlorobenzaldehyde + NADH + H+
4-chlorobenzyl alcohol + NAD+
-
-
-
-
r
4-deoxy-L-erythro-5-hexoseulose + NAD+
2-dehydro-3-deoxy-D-gluconate + NADH + H+
4-ethylcyclohexanol + NAD+
4-ethylcyclohexanone + NADH + H+
60% activity compared to cyclohexanol
-
-
r
4-ethylcyclohexanone + NADH + H+
? + NAD+
22% activity compared to cyclohexanone
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
4-hydroxy-2-butanone + NADH + H+
2,4-dihydroxybutane + NAD+
-
26% of the activity with phenyl trifluoromethyl ketone
-
-
?
4-hydroxy-retinol + NAD+
4-oxo-retinal + NADH
-
-
-
-
r
4-hydroxymethylpyrene + NAD+
4-formylpyrene + NADH + H+
-
-
-
r
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthol + NADH + H+
-
fluorogenic substrate of class I and II isozymes
-
-
?
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthyl alcohol + NADH + H+
-
substrate for class I ADH
-
-
?
4-methoxy-1-naphthaldehyde + NADH + H+
4-methoxynaphthalene-1-carbaldehyde + NAD+
-
substrate for class I ADH
-
-
r
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzyl alcohol + NAD+
-
51% of the activity with butan-2-ol
-
?
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxyphenylacetone + NADH
(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
-
-
?
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH + H+
56% activity compared to cyclohexanol
-
-
r
4-methylcyclohexanone + NADH + H+
4-methylcyclohexanol + NAD+
60% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
4-methylcyclohexanone + NADH + H+
? + NAD+
25% activity compared to cyclohexanone
-
-
?
4-methylpentan-2-one + NADH + H+
4-methyl-2-pentanol + NAD+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzylalcohol + NAD+
-
-
-
-
?
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
94% activity compared to benzyl alcohol
-
-
?
4-nitrosodimethylaniline + NAD+
? + NADH + H+
-
photometric assay substrate
-
-
?
4-nitrotetrazolium violet + NADH+ H+
a formazan + NAD+
4-phenylbutan-2-one + NADH
4-phenylbutan-2-ol + NAD+
-
-
-
-
?
5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+
-
-
-
-
r
5-acetylfuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
5-amino-1-pentanol + NAD+
delta-valerolactam + NADH + H+
38% conversion
-
-
?
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
5-hydroxymethylfurfural + NADH + H+
5-hydroxymethylfurfuryl alcohol + NAD+
low activity
-
-
ir
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
5alpha-pregnan-3beta-ol-20-one + NAD+
5alpha-pregnan-3,20-dione + NADH
-
low activity
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-cholanic acid-3-one + NADH
5beta-cholanic acid-3-ol + NAD+
-
low activity
-
-
?
5beta-cholanic acid-3-one + NADH + H+
5beta-cholanic acid-3-ol + NAD+
-
-
-
-
?
5beta-pregnan-21-ol-3,20-dione hemisuccinate + NAD+
5beta-pregnan-3,20,21-trione hemisuccinate + NADH + H+
-
-
-
-
?
5beta-pregnan-3,20-dione + NADH
?
-
-
-
-
?
5beta-pregnan-3beta-ol-20-one + NAD+
5beta-pregnan-3,20-dione + NADH
-
-
-
-
?
6-amino-1-hexanol + NAD+
epsilon-caprolactam + NADH + H+
14% conversion
-
-
?
6-benzyloxy-3,5-dioxo-hexanoic acid ethyl ester + NADH + H+
(3R,5S)-6-benzyloxy-3,5-dihydroxy-hexanoic acid ethyl ester + NAD+
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
7-cis-retinol + NAD+
7-cis-retinal + NADH
9-cis-retinol + NAD+
9-cis-retinal + NADH
a primary alcohol + NAD+
an aldehyde + NADH + H+
-
ADH3 is involved in multiple cellular pathways, as diverse as formaldehyde detoxification, retinoid metabolism and NO homeostasis, ADH3 is considered to play only a minor role in hepatic alcohol metabolism because ethanol concentrations rarely exceed 50 mM
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
acetaldehyde + NADPH + H+
ethanol + NADP+
acetoin + NAD+
diacetyl + NADH
18% of the activity with 2,3-butanediol
-
-
?
acetoin + NADH + H+
2,3-butanediol + NAD+
-
-
-
r
acetoin + NADH + H+
3-hydroxy-2-butanol + NAD+
-
-
-
-
r
acetoin + NADH + H+
butan-2,3-diol + NAD+
-
-
-
?
acetone + NADH
isopropanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
-
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
acetone + NADPH + H+
propan-2-ol + NADP+
14.7% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetophenone + NADH
(R)-alpha-phenyl ethanol + NAD+
-
the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion
-
-
?
acetophenone + NADH + H+
(R)-1-phenylethanol + NAD+
-
6% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 87% of the activity with 2-propanol
99% enantiomeric excess
-
?
acetophenone + NADH + H+
(S)-(-)-1-phenylethanol + NAD+
-
more than 99% enantiomeric excess
-
-
r
acetophenone + NADH + H+
(S)-1-phenylethanol + NAD+
-
-
99% enantiomeric excess
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADPH + H+
1-phenylethanol + NADP+
5.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetylacetone + 2 NADH + 2 H+
2,4-pentanediol + 2 NAD+
-
-
-
-
r
acetylacetone + NADH + H+
4-hydroxypentan-2-one + NAD+
1% activity compared to cyclohexanone
1% activity compared to cyclohexanone
-
r
acetylacetone + NADH + H+
?
-
63% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
acetylpyrazine + NADH + H+
(R)-1-(pyrazyl)ethanol + NAD+
-
-
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
all-trans retinal + NADH + H+
all-trans retinol + NAD+
26.8% of the activity with butan-1-ol
-
-
?
all-trans-retinal + NADH + H+
all-trans-retinol + NAD+
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
allyl alcohol + NAD+
acrolein + NADH
allyl alcohol + NAD+
prop-2-en-1-al + NADH
allylalcohol + NAD+
prop-2-enal + NADH + H+
-
-
-
?
alpha-ethyl benzoylformate + NADH + H+
ethyl (R)-(-)-mandelate + NAD+
-
-
95% enantiomeric excess
-
r
alpha-methyl benzoylformate + NADH + H+
methyl (R)-mandelate + NAD+
-
-
92% enantiomeric excess
-
r
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
alpha-tetralol + NAD+
-
-
-
-
r
anisaldehyde + NADH + H+
anisic alcohol + NAD+
-
-
-
-
?
benzacetaldehyde + NADPH + H+
benzol + NADP+
low activity, reaction of EC 1.1.1.2
-
-
ir
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
107% of the activity with (2E)-but-2-enal, yield 99% after 12 h
-
-
r
benzil + NADH + H+
(R)-benzoin + NAD+
62% of the activity compared to 1-phenyl-1,2-propanedione. The enzyme catalyses the asymmetric reduction of benzil to (R)-benzoin with both excellent conversion (98%) and optical purity (98%) by way of an efficient in situ NADH-recycling system involving a second thermophilic ADH
-
-
?
benzoin + NADH + H+
1,2-diphenylethane-1,2-diol
-
13% of activity with N-benzyl-3-pyrrolidinone
-
-
?
benzoylformic acid + NADPH + H+
mandelate + NADP+
2.4% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
benzyl alcohol + acycloNAD+
benzaldehyde + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
-
-
r
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
benzylalcohol + NAD+
benzaldehyde + NADH + H+
benzyloxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+
-
production with 99.6% enantiomeric excess
-
-
?
butan-1-ol + acycloNAD+
butanal + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
butan-1-ol + NAD+
butanal + NADH + H+
butan-2,3-diol + NAD+
acetoin + NADH + H+
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
butan-2-ol + NAD+
butanone + NADH + H+
-
83% of the activity with butan-2-ol
-
?
butan-2-one + NADH
butan-2-ol + NAD+
butanal + NADH
1-butanol + NAD+ + H+
-
% of the activity with phenyl trifluoromethyl ketone
-
-
ir
butanal + NADH
n-butanol + NAD+
butanal + NADH + H+
butan-1-ol + NAD+
-
-
-
r
butanol + NAD+
1-butanal + NADH + H+
about 15% of the activity with ethanol or 1-propanol
-
-
?
butanol + NAD+
butanal + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
n-butanal + NADH
-
-
-
?
butyraldehyde + NAD+
n-butanol + NADH + H+
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
-
56% of the activity with acetaldehyde
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADPH + H+
butanol + NADP+
-
22% of the activity with NADH
-
-
?
capronaldehyde + NADH
1-hexanol + NAD+
chloroacetone + NADH + H+
1-chloro-2-propanol + NAD+
-
-
-
-
r
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
choline + NAD+
? + NADH + H+
3% activity compared to benzyl alcohol
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH + H+
-
-
-
-
r
cinnamyl alcohol + NAD+
cinnamyl aldehyde + NADH + H+
-
-
-
?
cis-4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
-
-
-
-
?
cis-decahydro-1-naphthol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
cycloheptanol + NAD+
cycloheptanone + NADH + H+
cycloheptanone + NADH
cycloheptanol + NAD+
cyclohexanol + NAD+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
?
-
activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
cyclooctanone + NADH
cyclooctanol + NAD+
-
-
-
-
?
cyclopentanol + NAD+
? + NADH
cyclopentanol + NAD+
cyclopentanone + NADH + H+
cyclopentanone + NADH
cyclopentanol + NAD+
-
-
-
-
?
cyclopentanone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
cyclopetanone + NADH + H+
cyclopentanol + NAD+
-
-
-
-
?
D-allonate + NADH + H+
?
10% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-altronate + NADH + H+
?
8% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-arabinose + NADH + H+
D-arabitol + NAD+
66% of the activity with 2,3-butanediol
-
-
?
D-arabitol + NADH + H+
D-lyxose + D-arabinose + NAD+
-
-
-
?
D-erythrose + NAD+
?
-
-
-
?
D-glucitol + NAD+
? + NADH
D-gluconate + NADH + H+
?
79% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-glyceraldehyde + NAD+
?
-
-
-
?
D-mannonate + NADH + H+
?
37% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-ribonate + NADH + H+
?
0.3% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-sorbitol + NADH + H+
L-gulose + NAD+
0.01% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-talonate + NADH + H+
?
3% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
D-threose + NAD+
?
-
-
-
?
D-xylonate + NADH + H+
?
1% activity compared to 2-dehydro-3-deoxy-D-gluconate
-
-
?
decahydro-2-naphthol + NAD+
?
37% activity compared to cyclohexanol
-
-
?
decan-1-ol + NAD+
decanal + NADH + H+
-
-
-
r
decyl aldehyde + NADH + H+
1-decanol + NAD+
21% activity compared to benzyl alcohol
-
-
?
diacetyl + 2 NADH + 2 H+
2,3-butandiol + 2 NAD+
-
-
-
-
r
diacetyl + NADH + H+
acetoin + NAD+
150% of the activity with acetoin
-
-
?
diethylketone + NADH + H+
3-pentanol + NAD+
-
-
-
-
r
digitose + NAD+
? + NADH
-
-
-
-
?
dihydro-4,4-dimethyl-2,3-furandione + NADH + H+
?
-
13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
dihydroxyacetone + NADH + H+
glycerol + NAD+
36% of the activity with acetoin
-
-
?
dihydroxyacetone phosphate + NADH + H+
glycerol phosphate + NAD+
82% of the activity with acetoin
-
-
?
DL-glyceraldehyde + H+
glycerol + NAD+
31% of the activity with acetoin
-
-
?
docosanol + NAD+
? + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 20% reaction rate with docosanol compared to ethanol at pH 8.8 and 30ưC
-
-
?
dodecan-1-ol + NAD+
dodecanal + NADH + H+
-
-
-
r
dodecanol + NAD+
dodecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 35% reaction rate with dodecanol compared to ethanol at pH 8.8 and 30ưC
-
-
?
eicosan-1-ol + NAD+
n-eicosanal + NADH + H+
-
-
-
r
ethanol + 3-benzoylpyridine-adenine dinucleotide
acetaldehyde + ?
-
rapid equilibrium bi bi mechanism
-
-
?
ethanol + acycloNAD+
acetaldehyde + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moietyhas been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
ethanol + beta-NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+
aldehyde + NADH + H+
-
immobilized HLAD shows 100% reaction rate and free HLAD shows about 55% reaction rate with ethanol at pH 8.8 and 30ưC
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
ethyl (2,4-dichlorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(2,4-dichlorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (3,5-difluorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(3,5-difluorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-bromophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-bromophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-chlorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-chlorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-cyanophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-cyanophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-fluorophenyl)(oxo)acetate + NADH + H+
ethyl (2R)-(4-fluorophenyl)(hydroxy)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl (4-methylphenyl)(oxo)acetate + NADH + H+
ethyl (2R)-hydroxy(4-methylphenyl)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
(R)-ethyl 2-hydroxypropanoate + NADP+
294& of the activity with acetoin
-
-
r
ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+
ethyl (2R)-2-hydroxy-3,3-dimethylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate
-
-
?
ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+
ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutanoate + NADH + H+
ethyl (2R)-2-hydroxy-3-methylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutanoate + NADH + H+
ethyl (2S)-2-hydroxy-3-methylbutanoate + NAD+
enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate
-
-
?
ethyl 3-methyl-2-oxobutyrate + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
ethyl 3-methyl-2-oxobutyrate + NADH + H+
ethyl-2-hydroxy-3-methylbutyrate + NAD+
-
33% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 3-oxo-3-phenylpropanoate + NADH + H+
ethyl (3S)-3-hydroxy-3-phenylpropanoate + NAD+
ethyl (3S)-3-hydroxy-3-phenylpropanoate is produced with 99% enantiomeric excess
-
-
?
ethyl 3-oxobutanoate + NADH
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl (3S)-3-hydroxybutanoate + NAD+
ethyl (3S)-3-hydroxybutanoate is produced with 95% enantiomeric excess
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
ethyl 3-oxobutyrate + NADH + H+
ethyl (S)-3-hydroxybutyrate + NAD+
-
-
-
-
?
ethyl 3-oxohexanoate + NADH
ethyl 3-hydroxyhexanoate + NAD+
-
-
-
-
?
ethyl 3-oxohexanoate + NADH + H+
ethyl (3R)-3-hydroxyhexanoate + NAD+
ethyl (3R)-3-hydroxyhexanoate is produced with 24% enantiomeric excess
-
-
?
ethyl 3-oxopentanoate + NADH
ethyl 3-hydroxypentanoate + NAD+
-
-
-
-
?
ethyl 3-oxopentanoate + NADH + H+
ethyl (3S)-3-hydroxypentanoate + NAD+
ethyl (3S)-3-hydroxypentanoate + NAD+is produced with 60% enantiomeric excess
-
-
?
ethyl 3-phenyl-3-oxopropanoate + NADH
ethyl 3-phenyl-3-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl 4-bromo-3-oxobutanoate + NADH + H+
ethyl 4-bromo-3-hydroxybutanoate
-
511% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl (3R)-4-chloro-3-hydroxybutanoate + NAD+
ethyl (3R)-4-chloro-3-hydroxybutanoate is produced with 4% enantiomeric excess
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
809% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH +H+
(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+
COBE, ADH performs a enantioselective reduction, reacting as an anti-Prelog reductase
-
-
r
ethyl 4-chloroacetoacetate + NADH + H+
?
-
6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl 4-chloroacetoacetate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl 4-chloroacetoacetate + NADPH
?
activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH
-
-
?
ethyl 4-methyl-3-oxopentanoate + NADH + H+
ethyl (3S)-3-hydroxy-4-methylpentanoate + NAD+
ethyl (3S)-3-hydroxy-4-methylpentanoate is produced with 16% enantiomeric excess
-
-
?
ethyl acetoacetate + NADH + H+
?
-
42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl acetoacetate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl benzoylformate + NADH
?
about 30% of the activity compared to isatin
-
-
?
ethyl benzoylformate + NADH
ethyl (R)-mandelate + NAD+
-
enantiomeric excess of 99.9%
-
-
?
ethyl benzoylformate + NADH + H+
?
23% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-(-)-mandelate + NAD+
-
95% enantiomeric excess
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
ethyl oxo(phenyl)acetate + NADH + H+
ethyl (2R)-hydroxy(phenyl)acetate + NAD+
the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters
-
-
?
ethyl oxo(phenyl)acetate + NADH + H+
ethyl hydroxy(phenyl)acetate + NAD+
-
100% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
ethyl pyruvate + NADH
ethyl 2-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropanoate + NAD+
-
488% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropionate + NAD+
16% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl pyruvate + NADH + H+
ethyl lactate + NAD+
-
-
-
-
r
ethyl trifluoroacetoacetate + NADPH + H+
1-ethoxy-2,2,2-trifluoroethanol + NADP+
4.3% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
ethylene glycol + NAD+
? + NADH
ethylenglycol + NAD+
? + NADH
-
-
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
furfural + NADH + H+
furfuryl alcohol + NAD+
furfuryl alcohol + NAD+
furfural + NADH
glutaraldehyde + NADH + H+
?
-
70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
glutaraldehyde + NADH + H+
? + NAD+
best substrate in reduction reaction
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
glycolaldehyde + NADH + H+
glycol + NAD+
heptaldehyde + NADH + H+
? + NAD+
33% activity compared to benzyl alcohol
-
-
?
heptan-2-ol + NAD+
heptan-2-one + NADH
-
-
-
-
?
heptan-4-ol + NAD+
heptan-4-one + NADH
-
-
-
-
?
hexadecan-1-ol + NAD+
hexadecanal + NADH + H+
-
-
-
r
hexadecanol + NAD+
hexadecanal + NADH
-
very low activity
-
-
?
hexadecanol + NAD+
hexadecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 15% reaction rate with hexadecanol compared to ethanol at pH 8.8 and 30ưC
-
-
?
hexaldehyde + NADH + H+
1-hexanol + NAD+
7% activity compared to benzyl alcohol
-
-
?
hexan-1-ol + acycloNAD+
hexanal + acycloNADH + H+
-
acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety
-
-
?
hexan-1-ol + NAD+
hexanal + NADH + H+
hexan-1-ol + NAD+
n-hexanal + NADH
hexan-2-ol + NAD+
2-hexanone + NADH
-
-
-
-
?
hexanal + NADH + H+
1-hexanol + NAD+
69.6% of the activity with glutaraldehyde
-
-
?
hexanal + NADH + H+
hexan-1-ol + NAD+
16% of the activity with acetoin
-
-
?
hexanal + NADH + H+
n-hexanol + NAD+
hexanal + NADPH + H+
1-hexanone + NADP+
-
24% of the activity with NADH
-
-
?
hexanol + NAD+
hexaldehyde + NADH
-
-
-
-
?
hexanol + NAD+
hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
hydrocinnamaldehyde + NADH + H+
hydrocinnamyl alcohol + NAD+
12% activity compared to benzyl alcohol
-
-
r
hydrocinnamyl alcohol + NAD+
hydrocinnamaldehyde + NADH + H+
31% activity compared to benzyl alcohol
-
-
r
hydroxyacetone + NADH + H+
propane-1,2-diol + NAD+
-
27% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
isatin + NADH + H+
? + NAD+
iso-propanol + NAD+
? + NADH + H+
-
-
-
?
iso-propanol + NAD+
isopropanal + NADH + H+
isoamylalcohol + NAD+
3-methylbutanal NADH + H+
-
-
-
r
isoborneol + NAD+
?
isoborneol is the best substrate in the oxidation reaction
-
-
?
isobutanal + NADH + H+
isobutanol + NAD+
20% of the activity with glutaraldehyde
-
-
?
isobutyl alcohol + NAD+
? + NADH
-
-
-
-
?
isobutyraldehyde + NADH + H+
isobutanol + NAD+
isobutyramide + NAD+
?
-
-
-
-
r
isopentan-1-ol + NAD+
isopentanal + NADH + H+
-
34% of the activity with ethanol
-
-
?
isopentanol + NAD+
? + NADH + H+
isopentenyl alcohol + NAD+
isopentanone + NADH
-
-
-
-
?
isopropanol + NAD+
2-propanone + NADH + H+
-
-
-
r
isopropanol + NAD+
acetone + NADH + H+
isopropanol + NAD+
propan-2-one + NADH + H+
-
-
-
r
L-glyceraldehyde + NAD+
?
-
-
-
?
levulinic acid + NADH + H+
?
-
11% of activity with N-benzyl-3-pyrrolidinone
-
-
?
m-chlorophenacyl chloride + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
m-nitrobenzaldehyde + NAD+
m-nitrobenzyl alcohol + NADH + H+
-
substrate of class IV ADH
-
-
?
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
mannitol + NAD+
? + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
methyl 4-bromo-3-oxobutanoate + NADH + H+
methyl 4-bromo-3-hydroxybutanoate + NAD+
-
164% of the activity with phenyl trifluoromethyl ketone
-
-
?
methyl 4-chloro-3-oxobutanoate + NADH + H+
methyl 4-chloro-3-hydroxybutanoate + NAD+
methyl acetoacetate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
methyl benzoylformate + NADH
?
methyl benzoylformate + NADH + H+
methyl (R)-(-)-mandelate + NAD+
-
92% enantiomeric excess
-
-
?
methyl benzoylformate + NADH + H+
methyl (S)-mandelate + NAD+
-
6 h, 100% conversion, 17% enantiomeric excess
-
r
methyl glyoxal + NADH + H+
?
-
3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl o-chlorobenzoylformate + NADH
?
about 55% of the activity compared to isatin
-
-
?
methyl o-chlorobenzoylformate + NADH + H+
methyl (R)-o-chloromandelate + NAD+
-
6 h, 99% conversion, 72% enantiomeric excess
-
r
methyl oxo(phenyl)acetate + NADH + H+
methyl hydroxy(phenyl)acetate + NAD+
-
57% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
methyl pyruvate + NADH + H+
methyl 2-hydroxypropanoate + NAD+
-
18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl pyruvate + NADH + H+
methyl lactate + NAD+
-
-
-
-
r
methylbenzoylformate + NADH + H+
?
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
methylcrotonyl alcohol + NAD+
methylcrotonaldehyde + NADH
-
-
-
-
?
methylglyoxal + NADH + H+
? + NAD+
-
11.9% of the activity with acetaldehyde
-
-
?
methylglyoxal + NADH + H+
acetol + NAD+
-
-
-
r
N-benzyl-3-piperidone + NADH + H+
N-benzylpiperidin-3-ol + NAD+
-
2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
N-benzyl-3-pyrrolidinol + NAD+
1-benzylpyrrolidin-3-one + NADH + H+
-
33% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
N-benzyl-3-pyrrolidinone + NADH + H+
(R)-N-benzyl-3-pyrrolidinol + NAD+
-
(R)-stereoselectivity of the reduction carried out with the heat-treated cells
-
-
r
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
N-benzyl-4-piperidone + NADH + H+
N-benzylpiperidin-4-ol + NAD+
-
10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-butanal + NADH + H+
n-butanol + NAD+
-
-
-
-
?
n-butanol + NAD+
butanal + NADH
-
low activity
-
-
?
n-butanol + NAD+
butylaldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
n-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
r
n-butanol + NAD+
n-butanal + NADH
n-butanol + NAD+
n-butanal + NADH + H+
-
-
-
-
?
n-butanol + NADH + H+
butyraldehyde + NAD+
-
-
-
r
n-butylaldehyde + NADH + H+
butanal + NAD+
-
activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-butyraldehyde + NADH + H+
n-butanol + NAD+
-
7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-decanol + NAD+
n-decanal + NADH
-
-
-
-
r
n-hexanol + NAD+
n-hexanal + NADH
-
-
-
-
r
n-hexylaldehyde + NADH + H+
hexanal + NAD+
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-octylaldehyde + NADH + H+
n-octanol + NAD+
-
activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-pentanol + NAD+
pentanal + NADH + H+
-
-
-
-
?
n-pentylaldehyde + NADH + H+
n-pentanol + NAD+
-
-
-
-
r
n-propanol + NAD+
n-propanal + NADH
-
-
-
-
r
n-propanol + NAD+
propanal + NADH
n-propanol + NAD+
propanal + NADH + H+
n-propanol + NAD+
propionaldehyde + NADH
-
-
-
-
?
N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+
-
production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess
-
-
?
n-valeraldehyde + NADH + H+
n-pentanol + NAD+
-
activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
N-[4-(chloroacetyl)-2-nitrophenyl]acetamide + NADH + H+
N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide + NAD+
AE010289.1
production of N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide with 98% enantiomeric excess
-
-
?
nonyl aldehyde + NADH + H+
1-nonanol + NAD+
25% activity compared to benzyl alcohol
-
-
?
nonylphenol polyethoxylate 9 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
octacosan-1-ol + NAD+
octacosanal + NADH + H+
-
-
-
r
octadecan-1-ol + NAD+
octadecanal + NADH + H+
-
-
-
r
octan-1-ol + NAD+
n-octanal + NADH
octan-1-ol + NAD+
octanal + NADH + H+
-
-
-
r
octan-2-ol + NAD+
octan-2-one + NADH
octanal + NADH + H+
octanol + NAD+
octanol + NAD+
octanal + NADH
octanol + NAD+
octanal + NADH + H+
octyl aldehyde + NADH + H+
1-octanol + NAD+
29% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 2 + NAD+
? + NADH
59% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 8 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
oxalacetic acid + NADH + H+
?
-
14% of activity with N-benzyl-3-pyrrolidinone
-
-
?
oxaloacetate + NADH + H+
? + NAD+
-
-
-
-
r
p-chlorobenzaldehyde + NADH + H+
p-chlorobenzyl alcohol + NAD+
-
1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
p-nitrobenzaldehyde + NADH
p-nitrobenzyl alcohol + NAD+
-
-
-
-
?
p-nitrobenzaldehyde + NADH + H+
p-nitrobenzyl alcohol + NAD+
-
substrate of isozyme ADH4