Application | Comment | Organism |
---|---|---|
synthesis | under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h | Kangiella koreensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Kangiella koreensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
- |
Kangiella koreensis |
40000 | - |
- |
Kangiella koreensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kangiella koreensis | C7R702 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79.6 | - |
substrate butan-1-ol, pH 6.5, 60°C | Kangiella koreensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | 83.2% of the activity with butan-1-ol | Kangiella koreensis | ethanol + NAD+ | - |
? | |
all-trans retinal + NADH + H+ | 26.8% of the activity with butan-1-ol | Kangiella koreensis | all-trans retinol + NAD+ | - |
? | |
butan-1-ol + NAD+ | - |
Kangiella koreensis | butanal + NADH + H+ | - |
? | |
hexan-1-ol + NAD+ | 77.1% of the activity with butan-1-ol | Kangiella koreensis | hexanal + NADH + H+ | - |
? | |
additional information | enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184 | Kangiella koreensis | ? | - |
? | |
propan-1-ol + NAD+ | 64.3% of the activity with butan-1-ol | Kangiella koreensis | propanal + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE, x * 39900, calculated | Kangiella koreensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Kangiella koreensis | |
NADH | - |
Kangiella koreensis |