Cloned (Comment) | Organism |
---|---|
expression of ADH4 in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
all-trans-retinal | product inhibition | Homo sapiens | |
all-trans-retinoic acid | weak feedback inhibition | Homo sapiens | |
Isobutyramide | substrate inhibition, competitive against retinol, noncompetitive against NADH | Homo sapiens | |
trifluoroethanol | competitive against retinol, noncompetitive against NAD+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, kinetic mechanism | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol + NAD+ | Homo sapiens | ADH4 might be involved in biosynthesis of retinoic acid | all-trans-retinal + NADH | - |
r | |
isobutyramide + NAD+ | Homo sapiens | - |
? | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
class IV alcohol dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by DEAE ion exchange, 5'-AMP-resin affinity, and Mono Q ion exchange chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a primary alcohol + NAD+ = an aldehyde + NADH + H+ | class IV alcohol dehydrogenase also functions as retinol dehydrogenase, reaction and kinetic mechanism: asymmetric rapid equilibrium random mechanism with 2 dead-end ternary complexes fro retinol oxidation and a rapid equilibrium ordered mechanism with one dead-end ternary complex for retinal reduction, a unique mechanistic form fro zinc-containing ADH in the medium chain dehydrogenase/reductase superfamily of enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol + NAD+ | - |
Homo sapiens | all-trans-retinal + NADH | - |
r | |
all-trans-retinol + NAD+ | ADH4 might be involved in biosynthesis of retinoic acid | Homo sapiens | all-trans-retinal + NADH | - |
r | |
isobutyramide + NAD+ | - |
Homo sapiens | ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADH4 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |