Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
G211C | mutant enzyme exhibits almost complete reversals in cofactor specificity | Pyrococcus furiosus |
G211InsA | with NADP+, the mutant enzyme shows a 29-fold increase in kcat as compared to the wild-type enzyme | Pyrococcus furiosus |
G211InsC | with NAD+, the mutant enzyme exhibits 6-fold decrease in kcat as compared to wild-type enzyme. Mutant enzyme exhibits almost complete reversals in cofactor specificity | Pyrococcus furiosus |
G211InsG | with NAD+, the mutant enzyme exhibits 2.5-fold enhancement in kcat over the wild-type enzyme. Activity with NADP+ exceeds that of the wild type enzyme | Pyrococcus furiosus |
G211InsS | with NAD+, the mutant enzyme exhibits 2.5-fold enhancement in kcat over the wild-type enzyme. With NADP+, the mutant enzyme shows a 29-fold increase in kcat as compared to the wild-type enzyme | Pyrococcus furiosus |
G211S | with NAD+, the mutant enzyme exhibits 1.5-fold enhancement in kcat over the wild-type. Activity with NADP+ exceeds that of the wild type enzyme | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsC | Pyrococcus furiosus | |
0.1 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211S | Pyrococcus furiosus | |
0.12 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsA | Pyrococcus furiosus | |
0.23 | - |
NAD+ | pH 8.8, 45°C, wild-type enzyme | Pyrococcus furiosus | |
0.29 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsG | Pyrococcus furiosus | |
0.44 | - |
2,3-Butanediol | pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+ | Pyrococcus furiosus | |
0.48 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211C | Pyrococcus furiosus | |
0.91 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+ | Pyrococcus furiosus | |
1.5 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+ | Pyrococcus furiosus | |
1.6 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsS | Pyrococcus furiosus | |
5.5 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+ | Pyrococcus furiosus | |
7.4 | - |
NADP+ | pH 8.8, 45°C, wild-type enzyme | Pyrococcus furiosus | |
9.6 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+ | Pyrococcus furiosus | |
12 | - |
2,3-Butanediol | pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+ | Pyrococcus furiosus | |
15 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211S | Pyrococcus furiosus | |
17 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+ | Pyrococcus furiosus | |
25 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211C | Pyrococcus furiosus | |
33 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsG | Pyrococcus furiosus | |
66 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsS | Pyrococcus furiosus | |
76 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+ | Pyrococcus furiosus | |
90 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+ | Pyrococcus furiosus | |
100 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+ | Pyrococcus furiosus | |
110 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+ | Pyrococcus furiosus | |
120 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsC | Pyrococcus furiosus | |
240 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+ | Pyrococcus furiosus | |
260 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+ | Pyrococcus furiosus | |
360 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsA | Pyrococcus furiosus | |
1100 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+ | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanediol + NAD+ | - |
Pyrococcus furiosus | acetoin + NADH + H+ | - |
? | |
2,3-butanediol + NADP+ | - |
Pyrococcus furiosus | acetoin + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhD | - |
Pyrococcus furiosus |
alcohol dehydrogenase D | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
NADP+ | pH 8.8, 45°C, wild-type enzyme | Pyrococcus furiosus | |
0.073 | - |
2,3-Butanediol | pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+ | Pyrococcus furiosus | |
0.29 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211S | Pyrococcus furiosus | |
0.29 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+ | Pyrococcus furiosus | |
0.43 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsC | Pyrococcus furiosus | |
0.43 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+ | Pyrococcus furiosus | |
0.52 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsG | Pyrococcus furiosus | |
0.52 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+ | Pyrococcus furiosus | |
0.86 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsC | Pyrococcus furiosus | |
0.86 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+ | Pyrococcus furiosus | |
0.92 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211C | Pyrococcus furiosus | |
0.92 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+ | Pyrococcus furiosus | |
1.1 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211C | Pyrococcus furiosus | |
1.1 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+ | Pyrococcus furiosus | |
1.7 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsA | Pyrococcus furiosus | |
1.7 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+ | Pyrococcus furiosus | |
2.1 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsA | Pyrococcus furiosus | |
2.1 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+ | Pyrococcus furiosus | |
2.1 | - |
NADP+ | pH 8.8, 45°C, mutant enzyme G211InsS | Pyrococcus furiosus | |
2.1 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+ | Pyrococcus furiosus | |
2.6 | - |
NAD+ | pH 8.8, 45°C, wild-type enzyme | Pyrococcus furiosus | |
2.6 | - |
2,3-Butanediol | pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+ | Pyrococcus furiosus | |
4 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211S | Pyrococcus furiosus | |
4 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+ | Pyrococcus furiosus | |
6.5 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsG | Pyrococcus furiosus | |
6.5 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+ | Pyrococcus furiosus | |
6.7 | - |
NAD+ | pH 8.8, 45°C, mutant enzyme G211InsS | Pyrococcus furiosus | |
6.7 | - |
2,3-Butanediol | pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+ | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrococcus furiosus | |
NADP+ | - |
Pyrococcus furiosus |