Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
expression in Escherichia coli | Moraxella sp. |
Protein Variants | Comment | Organism |
---|---|---|
A25Y | mutation in the dimer-dimer interface, leads to a more thermostable enzyme and a change in the rate-determining step at low temperature | Moraxella sp. |
W87A | mutation results in a loss of the Arrhenius break seen at 30°C for the wild-type enzyme and an increase in cold lability due to destabilization of the active tetrameric form. Kinetic isotope effects are nearly temperature-independent over the experimental temperature range, and similar in magnitude to those measured above 30°C for the wild-type enzyme | Geobacillus stearothermophilus |
Y25A | mutation in the dimer-dimer interface, results in kinetic behavior similar to that of mutantion W87A | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | mutant W87A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
0.5 | - |
NAD+ | mutant A25Y, pH 7.0, 30°C | Moraxella sp. | |
1 | - |
NAD+ | mutant Y25A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
1.1 | - |
NAD+ | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
1.5 | - |
benzyl alcohol | mutant W87A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
6.9 | - |
benzyl alcohol | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
7.2 | - |
benzyl alcohol | mutant Y25A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
16 | - |
benzyl alcohol | mutant A25Y, pH 7.0, 30°C | Moraxella sp. | |
29.4 | - |
benzyl alcohol | wild-type, pH 7.0, 30°C | Moraxella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P42328 | - |
- |
Moraxella sp. | Q8GIX7 | - |
- |
Moraxella sp. TAE123 | Q8GIX7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | - |
Geobacillus stearothermophilus | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Moraxella sp. | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Moraxella sp. TAE123 | benzaldehyde + NADH + H+ | - |
? | |
additional information | mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away | Geobacillus stearothermophilus | ? | - |
? | |
additional information | mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away | Moraxella sp. | ? | - |
? | |
additional information | mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away | Moraxella sp. TAE123 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
benzyl alcohol | mutant A25Y, pH 7.0, 30°C | Moraxella sp. | |
5.5 | - |
benzyl alcohol | mutant W87A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
6.9 | - |
benzyl alcohol | wild-type, pH 7.0, 30°C | Moraxella sp. | |
14 | - |
benzyl alcohol | mutant Y25A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
24.9 | - |
benzyl alcohol | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NAD+ | - |
Moraxella sp. |