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Literature summary for 1.1.1.1 extracted from
- Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase (2009), Appl. Environ. Microbiol., 75, 1758-1763.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfurisphaera tokodaii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | the enzyme likely contains 2 Zn2+ | Sulfurisphaera tokodaii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37477 | - |
4 * 37477, calculeted from sequence | Sulfurisphaera tokodaii |
| 38000 | - |
4 * 38000, SDS-PAGE | Sulfurisphaera tokodaii |
| 133000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | F9VMI9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.14 | - |
pH 10.5, 50°C | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hexanol + NAD+ | - |
Sulfurisphaera tokodaii | hexanal + NADH + H+ | - |
? | |
| benzaldehyde + NADH | - |
Sulfurisphaera tokodaii | benzyl alcohol + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 38000, SDS-PAGE | Sulfurisphaera tokodaii |
| homotetramer | 4 * 37477, calculeted from sequence | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD-dependent medium-chain ADH | - |
Sulfurisphaera tokodaii |
| ST0053 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Sulfurisphaera tokodaii |
| 85 | - |
- |
Sulfurisphaera tokodaii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | 70°C: about 65% of maximal activity, 95°C: about 80% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, stable. 120 min, 50% loss of activity | Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reduction of benzaldehyde | Sulfurisphaera tokodaii |
| 10.5 | - |
oxidation of 1-hexanol | Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | pH 5.5: about 35% of maximal activity, pH 7.5: about 55% of maximal activity, reduction of benzaldehyde | Sulfurisphaera tokodaii |
| 7.5 | 10.8 | pH 7.5: about 35% of maximal activity, pH 10.8: about 50% of maximal activity, oxidation of 1-hexanol | Sulfurisphaera tokodaii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | 30 min, 50°C, 50% loss of activity | Sulfurisphaera tokodaii |
| 3.3 | - |
30 min, 50°C, 50% loss of activity | Sulfurisphaera tokodaii |
| 4 | 10 | 30 min, 50°C, no loss of activity | Sulfurisphaera tokodaii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sulfurisphaera tokodaii | |
| NADH | - |
Sulfurisphaera tokodaii | |
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