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Literature summary for 1.1.1.1 extracted from

  • Machielsen, R.; Uria, A.R.; Kengen, S.W.; van der Oost, J.
    Production and characterization of a thermostable alcohol dehydrogenase that belongs to the aldo-keto reductase uperfamily (2006), Appl. Environ. Microbiol., 72, 233-238.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 NADH pH 6.1, 70°C Pyrococcus furiosus
0.6
-
 NAD+ pH 8.8, 70°C Pyrococcus furiosus
6.5
-
 acetoin reduction reaction, pH 6.1, 70°C Pyrococcus furiosus
86.8
-
 Butan-2,3-diol pH 8.8, 70°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Pyrococcus furiosus
32000
-
 1 * 32000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZM9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2 2.5 substrate diacetyl-acetoin, maximal specific activity detected in reduction reaction, 70°C, pH 6.1 Pyrococcus furiosus
108
-
 substrate butan-2,3-diol, maximal specific activity detected in oxidation reaction, 70°C, pH 8.8 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoin + NAD+ 18% of the activity with 2,3-butanediol Pyrococcus furiosus diacetyl + NADH
-
 ?
acetoin + NADH + H+
-
 Pyrococcus furiosus butan-2,3-diol + NAD+
-
 ?
butan-2,3-diol + NAD+
-
 Pyrococcus furiosus acetoin + NADH + H+
-
 ?
cyclohexanol + NAD+ 12% of the activity with 2,3-butanediol Pyrococcus furiosus cyclohexanone + NADH + H+
-
 r
cyclohexanone + NADH + H+ 38% of the activity with acetoin Pyrococcus furiosus cyclohexanol + NAD+
-
 r
D-arabinose + NADH + H+ 66% of the activity with 2,3-butanediol Pyrococcus furiosus D-arabitol + NAD+
-
 ?
diacetyl + NADH + H+ 150% of the activity with acetoin Pyrococcus furiosus acetoin + NAD+
-
 ?
dihydroxyacetone + NADH + H+ 36% of the activity with acetoin Pyrococcus furiosus glycerol + NAD+
-
 ?
dihydroxyacetone phosphate + NADH + H+ 82% of the activity with acetoin Pyrococcus furiosus glycerol phosphate + NAD+
-
 ?
DL-glyceraldehyde + H+ 31% of the activity with acetoin Pyrococcus furiosus glycerol + NAD+
-
 ?
hexanal + NADH + H+ 16% of the activity with acetoin Pyrococcus furiosus hexan-1-ol + NAD+
-
 ?
additional information broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols Pyrococcus furiosus ?
-
 ?
pentan-2-ol + NAD+
-
 Pyrococcus furiosus pentan-2-one + NADH + H+ 9% of the activity with 2,3-butanediol ?
pyruvaldehyde + NADH + H+ 81% of the activity with acetoin Pyrococcus furiosus lactaldehyde + NAD+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
AdhD
-
 Pyrococcus furiosus
PF1960
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life 130 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
 reduction of ketones Pyrococcus furiosus
8.8
-
 oxidation of secondary alcohols Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred over NADP+ Pyrococcus furiosus
NADP+ 14% of the activity with NAD+ Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 Butan-2,3-diol pH 8.8, 70°C Pyrococcus furiosus