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Literature summary for 1.1.1.1 extracted from

  • Tsuji, K.; Yoon, K.S.; Ogo, S.
    Biochemical characterization of a bifunctional acetaldehyde-alcohol dehydrogenase purified from a facultative anaerobic bacterium Citrobacter sp. S-77 (2016), J. Biosci. Bioeng., 121, 253-258 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene adhE, sequence comparisons Citrobacter sp. S-77

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Citrobacter sp. S-77
7.43
-
acetaldehyde pH 7.0, 30°C Citrobacter sp. S-77
460
-
ethanol pH 7.0, 30°C Citrobacter sp. S-77

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Citrobacter sp. S-77 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Citrobacter sp. S-77
-
acetaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Citrobacter sp. S-77
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from plasma membrane by ultracentrifugation, hydroxyapatite and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration Citrobacter sp. S-77

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
purified native enzyme, pH 7.0, 30°C, ethanol oxidation with NAD+ Citrobacter sp. S-77

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Citrobacter sp. S-77 acetaldehyde + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
acetaldehyde-alcohol dehydrogenase
-
Citrobacter sp. S-77
AdhE
-
Citrobacter sp. S-77
ADHES77
-
Citrobacter sp. S-77
bifunctional acetaldehyde-alcohol dehydrogenase
-
Citrobacter sp. S-77

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Citrobacter sp. S-77

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
acetaldehyde pH 7.0, 30°C Citrobacter sp. S-77
3.2
-
ethanol pH 7.0, 30°C Citrobacter sp. S-77

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Citrobacter sp. S-77

Cofactor

Cofactor Comment Organism Structure
additional information the purified ADHES77 prefers to use NAD+ as an electron acceptor as compared to NADP+. In the specific activity of ethanol dehydrogenase, the catalytic efficiency for NAD+ is about 56.7fold greater than for NADP+. Similarly, in the catalytic reduction of acetaldehyde, the ADHES77 preferentially utilizes NADH as an electron donor, exhibiting approximately 12.5fold higher activity, than for NADPH. The results suggest that the ADHES77 prefers to use NAD(H) as redox partners as compared to NADP(H) Citrobacter sp. S-77
NAD+ preferred cofactor Citrobacter sp. S-77
NADH preferred cofactor Citrobacter sp. S-77
NADP+
-
Citrobacter sp. S-77
NADPH
-
Citrobacter sp. S-77

General Information

General Information Comment Organism
additional information the enzyme's two functional domains are fused into a single polypeptide by a linker amino acid region Citrobacter sp. S-77
physiological function acetaldehyde-alcohol dehydrogenase (ADHE) is a bifunctional enzyme consisting of two domains of an N-terminal acetaldehyde dehydrogenase (ALDH) and a C-terminal alcohol dehydrogenase (ADH). The N-terminal domain is responsible for the conversion of acetyl-CoA to acetaldehyde and the C-terminal domain is subsequently responsible for the conversion of acetaldehyde to ethanol. The enzyme is important in the cellular alcohol metabolism. The coenzyme A-acylating ADHE from Citrobacter sp. S-77 may play a pivotal role in modulating intracellular acetaldehyde concentration Citrobacter sp. S-77

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.007
-
ethanol pH 7.0, 30°C Citrobacter sp. S-77
0.336
-
acetaldehyde pH 7.0, 30°C Citrobacter sp. S-77