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Literature summary for 1.1.1.1 extracted from

  • Yabe, M.; Shitara, K.; Kawashima, J.; Shinoyama, H.; Ando, A.; Fujii, T.
    Purification and properties of an alcohol dehydrogenase isozyme from a methanol-using yeast, Candida sp. N-16 (1992), Biosci. Biotechnol. Biochem., 56, 338-339.
No PubMed abstract available
Search for more literature for 1.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA enhances activity Candida sp. (in: Saccharomycetales)

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 0.1 mM, 20% decrease of activity Candida sp. (in: Saccharomycetales)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.195
-
 NAD+
-
 Candida sp. (in: Saccharomycetales)
0.487
-
 NAD+
-
 Candida sp. (in: Saccharomycetales)

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enhances activity Candida sp. (in: Saccharomycetales)
Mg2+ enhances activity Candida sp. (in: Saccharomycetales)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Candida sp. (in: Saccharomycetales)
180000
-
 gel filtration Candida sp. (in: Saccharomycetales)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida sp. (in: Saccharomycetales) one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII ?
-
 ?
additional information Candida sp. (in: Saccharomycetales) N-16 one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida sp. (in: Saccharomycetales)
-
 N-16
-
Candida sp. (in: Saccharomycetales) N-16
-
 N-16
-

Purification (Commentary)

Purification (Comment) Organism
ADH-MII Candida sp. (in: Saccharomycetales)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.3
-
-
 Candida sp. (in: Saccharomycetales)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADH + H+
-
 Candida sp. (in: Saccharomycetales) ethanol + NAD+
-
 ?
acetaldehyde + NADH + H+
-
 Candida sp. (in: Saccharomycetales) N-16 ethanol + NAD+
-
 ?
ethanol + NAD+ the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol Candida sp. (in: Saccharomycetales) acetaldehyde + NADH
-
 r
ethanol + NAD+ the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol Candida sp. (in: Saccharomycetales) N-16 acetaldehyde + NADH
-
 r
methanol + NAD+ reaction is extremly weak Candida sp. (in: Saccharomycetales) formaldehyde + NADH + H+
-
 ?
methanol + NAD+ reaction is extremly weak Candida sp. (in: Saccharomycetales) N-16 formaldehyde + NADH + H+
-
 ?
additional information one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII Candida sp. (in: Saccharomycetales) ?
-
 ?
additional information one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII Candida sp. (in: Saccharomycetales) N-16 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Candida sp. (in: Saccharomycetales)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 above Candida sp. (in: Saccharomycetales)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Candida sp. (in: Saccharomycetales)

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Candida sp. (in: Saccharomycetales)
NADH
-
 Candida sp. (in: Saccharomycetales)